pBAD TOPO® TA Expression Kit

Catalog number: K430001

Invitrogen™  Related applications: Protein Expression

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Description

The pBAD TOPO® TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products. Once you've TOPO® Cloned your PCR product, you can go straight to protein expression. Some of the convenient features of the pBAD-TOPO® vector include:

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• The araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with an Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Expression: E. coli
Promoter: araBAD
Bacterial or Yeast Strain: TOP10, LMG194
Cleavage: EK (Enterokinase) Recognition Site
Vector Type: pBAD
Product Size: 20 reactions
Cloning Method: TOPO®-TA
Inducing Agent: Arabinose
Expression Mechanism: Cell-Based Expression
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

Each pBAD TOPO® TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO® TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO® vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot® box contains all of the reagents necessary for transformation, including single-use 50 µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

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