Autoregulation of pituitary corticotroph SOCS-3 expression: characterization of the murine SOCS-3 promoter.
AuthorsAuernhammer CJ, Bousquet C, Melmed S
JournalProc Natl Acad Sci U S A
PubMed ID10359822
'Pituitary corticotroph SOCS-3 is a novel intracellular regulator of leukemia inhibitory factor (LIF)-mediated proopiomelanocortin gene expression and adrenocorticotropic hormone (ACTH) secretion, inhibiting LIF-activated Janus kinase-signal transducers and activators of transcription (STAT) signaling in a negative autoregulatory loop. We now demonstrate in corticotroph AtT-20 cells that LIF-stimulated endogenous SOCS-3 mRNA expression ... More
Single base mismatches in the mRNA target site allow specific seed region-mediated off-target binding of siRNA targeting human coagulation factor 7.
AuthorsRavon M, Berrera M, Ebeling M, Certa U
JournalRNA Biol
PubMed ID22258146
'We have analyzed the off-target activity of two siRNAs (F7-1, F7-2) that knock-down human blood coagulation factor 7 mRNA. F7-1 modulates a significant number of non-target transcripts while F7-2 shows high selectivity for the target transcript under various experimental conditions. The 3''-UTRs of all F7-1 off-target genes show statistically significant ... More
A host-specific function is required for ligation of a wide variety of ribozyme-processed RNAs.
AuthorsReid CE, Lazinski DW
JournalProc Natl Acad Sci U S A
PubMed ID10618434
'Hepatitis delta virus (HDV) replicates its circular RNA genome via a rolling circle mechanism. During this process, cis-acting ribozymes cleave adjacent upstream sequences and thereby resolve replication intermediates to unit-length RNA. The subsequent ligation of these 5''OH and 2'',3''-cyclic phosphate termini to form circular RNA is an essential step in ... More
Characterization of a Ustilago maydis gene specifically induced during the biotrophic phase: evidence for negative as well as positive regulation.
AuthorsBasse CW, Stumpferl S, Kahmann R.
JournalMol Cell Biol
PubMed ID10594035
The phytopathogenic basidiomycete Ustilago maydis requires its host plant, maize, for completion of its sexual cycle. To investigate the molecular events during infection, we used differential display to identify plant-induced U. maydis genes. We describe the U. maydis gene mig1 (for ... More
Genetic engineering of phytochrome biosynthesis in bacteria.
Authors Gambetta G A; Lagarias J C;
JournalProc Natl Acad Sci U S A
PubMed ID11553807
The bilin prosthetic groups of the phytochrome photoreceptors and the light-harvesting phycobiliprotein antennae arise from the oxygen-dependent ring opening of heme. Two ferredoxin-dependent enzymes contribute to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity. Using a dual plasmid system, one expressing a truncated cyanobacterial apophytochrome ... More
Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase.
AuthorsHetman JM, Soderling SH, Glavas NA, Beavo JA
JournalProc Natl Acad Sci U S A
PubMed ID10618442
A member of the phosphodiesterase (PDE)7 family with high affinity and specificity for cAMP has been identified. Based on sequence homologies, we designate this PDE as PDE7B. The full-length cDNA of PDE7B is 2399 bp, and its ORF sequence predicts a protein of 446 amino acids with a molecular mass ... More
Structural characterization of the PIT-1/ETS-1 interaction: PIT-1 phosphorylation regulates PIT-1/ETS-1 binding.
Authors Augustijn Kevin D.; Duval Dawn L.; Wechselberger Rainer; Kaptein Rob; Gutierrez-Hartmann Arthur; Van Der Vliet Peter C.;
JournalProc Natl Acad Sci U S A
PubMed ID12242337
The POU-domain transcription factor Pit-1 and Ets-1, a member of the ETS family of transcription factors, can associate in solution and synergistically activate the prolactin promoter by binding to a composite response element in the prolactin promoter. We mapped the minimal region of Ets-1 required for the interaction with the ... More
Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis.
Authors Stathopoulos C; Kim W; Li T; Anderson I; Deutsch B; Palioura S; Whitman W; Söll D;
JournalProc Natl Acad Sci U S A
PubMed ID11717392
The methanogenic archaea Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus contain a dual-specificity prolyl-tRNA synthetase (ProCysRS) that accurately forms both prolyl-tRNA (Pro-tRNA) and cysteinyl-tRNA (Cys-tRNA) suitable for in vivo translation. This intriguing enzyme may even perform its dual role in organisms that possess a canonical single-specificity cysteinyl-tRNA synthetase (CysRS), raising the question ... More
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
Authors Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;
JournalProc Natl Acad Sci U S A
PubMed ID11562501
Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More
Internal IgH class switch region deletions are position-independent and enhanced by AID expression.
Authors Dudley Darryll D; Manis John P; Zarrin Ali A; Kaylor Lianne; Tian Ming; Alt Frederick W;
JournalProc Natl Acad Sci U S A
PubMed ID12114543
Ig heavy chain class switch recombination (CSR) involves a recombination/deletion mechanism that exchanges the expressed C(H) gene with a downstream C(H) gene. CSR is mediated by highly repetitive switch (S) region sequences and requires the activation-induced deaminase (AID). The S region 5' of the C mu gene (S mu) can ... More
Synergistic effects of 2A-mediated polyproteins on the production of lignocellulose degradation enzymes in tobacco plants.
AuthorsLee DS, Lee KH, Jung S, Jo EJ, Han KH, Bae HJ
JournalJ Exp Bot
PubMed ID22798663
Cost-effective bioethanol production requires a supply of various low-cost enzymes that can hydrolyse lignocellulosic materials consisting of multiple polymers. Because plant-based enzyme expression systems offer low-cost and large-scale production, this study simultaneously expressed ß-glucosidase (BglB), xylanase (XylII), exoglucanase (E3), and endoglucanase (Cel5A) in tobacco plants, which were individually fused with ... More
Disruption of Abi1/Hssh3bp1 expression induces prostatic intraepithelial neoplasia in the conditional Abi1/Hssh3bp1 KO mice.
AuthorsXiong X, Chorzalska A, Dubielecka PM, White JR, Vedvyas Y, Hedvat CV, Haimovitz-Friedman A, Koutcher JA, Reimand J, Bader GD, Sawicki JA, Kotula L
JournalOncogenesis
PubMed ID23552839
Prostate cancer is one of the leading causes of cancer-related deaths in the United States and a leading diagnosed non-skin cancer in American men. Genetic mutations underlying prostate tumorigenesis include alterations of tumor suppressor genes. We tested the tumor suppressor hypothesis for ABI1/hSSH3BP1 by searching for gene mutations in primary ... More