TOPO® TA Cloning® Kit for Subcloning, with TOP10F' E. coli

Catalog number: K455040

Invitrogen™  Related applications: PCR Cloning

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


For fast, efficient subcloning in a variety of applications, the only choice is Invitrogen's TOPO® TA cloning Kit with our popular pCR™2.1 vector. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.

Convenient features of the TOPO® TA cloning Kit with our pCR2.1®-TOPO® TA vector are:3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter and M13 forward and reverse primer sites for in vitro RNA transcription and sequencing
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• 15 Convenient and validate restriction sites flanking your insert for easy, directional sub-cloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot® TOP10 F' Competent cells for added convenience and highest cloning efficiency
For Research Use Only. Not for use in diagnostic procedures.


Vector Type: TOPO-TA Cloning Vectors
Cloning Method: TOPO®-TA
Bacterial or Yeast Strain: TOP10F´
Product Size: 50 reactions

Contents & storage

Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO® vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.


Manuals & protocols

Recommended products