TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli

Catalog number:  K455040

 Related applications:

PCR Cloning

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For fast, efficient subcloning in a variety of applications, the only choice is Invitrogen's TOPO® TA cloning Kit with our popular pCR™2.1 vector. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.

Convenient features of the TOPO® TA cloning Kit with our pCR2.1®-TOPO® TA vector are:3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter and M13 forward and reverse primer sites for in vitro RNA transcription and sequencing
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• 15 Convenient and validate restriction sites flanking your insert for easy, directional sub-cloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot® TOP10 F' Competent cells for added convenience and highest cloning efficiency
For Research Use Only. Not for use in diagnostic procedures.


Vector Type: TOPO-TA Cloning Vectors
Cloning Method: TOPO®-TA
Bacterial or Yeast Strain: TOP10F´
Product Line: One Shot™
Product Size: 50 reactions

Contents & storage

Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO® vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot® Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.


Manuals & protocols