Identification of a Differentially Expressed Oligopeptide Binding Protein (OppA2) in Streptococcus uberis by Representational Difference Analysis of cDNA.
Streptococcus uberis is an increasingly significant cause of intramammary infection in the dairy cow, presently responsible for approximately 33% of all cases of bovine mastitis in the United Kingdom. Following experimentally induced infection of the lactating mammary gland, S. uberis is found predominantly in the luminal areas of secretory alveoli ... More
Characterization of a Novel Drosophila melanogaster Galectin. EXPRESSION IN DEVELOPING IMMUNE, NEURAL, AND MUSCLE TISSUES.
Authors Pace Karen E; Lebestky Tim; Hummel Thomas; Arnoux Pascal; Kwan Kent; Baum Linda G;
JournalJ Biol Chem
PubMed ID11809773
'We have cloned and characterized the first galectin to be identified in Drosophila melanogaster. The amino acid sequence of Drosophila galectin showed striking sequence similarity to invertebrate and vertebrate galectins and contained amino acids that are crucial for binding beta-galactoside sugars. Confirming its identity as a galectin family member, the ... More
Cardiac troponin T variants produced by aberrant splicing of multiple exons in animals with high instances of dilated cardiomyopathy.
Authors Biesiadecki Brandon J; Elder Benjamin D; Yu Zhi-Bin; Jin Jian-Ping;
JournalJ Biol Chem
PubMed ID12377784
'Adult cardiac muscle normally expresses a single cardiac troponin T (cTnT). As a potential pathogenic mechanism for turkey dilated cardiomyopathy, the splice-out of a normally constitutive exon generates an additional low molecular weight cTnT with altered conformation and function. We further found that aberrant splicing of cTnT also occurs in ... More
Molecular cloning and characterization of Foxp3 in Atlantic salmon (Salmo salar).
AuthorsZhang Z, Chi H, Niu C, Bøgwald J, Dalmo RA,
JournalFish Shellfish Immunol
PubMed ID21276855
'Foxp3 is a T cell-specific transcription factor and plays a key role in the development of Treg cells and in the immune regulatory process during inflammation. Here we report cloning and characterization of the full-length cDNA of Atlantic salmon Foxp3, which possesses a Forkhead domain, a zinc finger domain and ... More
Alternative splicing of the primary transcript generates heterogeneity within the products of the gene for Bombyx mori chitinase.
AuthorsAbdel-Banat BM, Koga D.
JournalJ Biol Chem
PubMed ID12045191
'The gene of chitinase in the silkworm, Bombyx mori, generates four mRNA products by alternative splicing. Nucleotide sequences of the entire gene for chitinase and respective cDNAs demonstrate that the pre-mRNA undergoes alternative splicing at both the 5'' and 3'' regions. At the 5'' region, the pre-mRNA experienced differential splicing ... More
Cloning and characterization of a lysosomal phospholipase A2, 1-o-acylceramide synthase.
Authors Hiraoka Miki; Abe Akira; Shayman James A;
JournalJ Biol Chem
PubMed ID11790796
'Recently, a novel enzyme, 1-O-acylceramide synthase (ACS), was purified and characterized from bovine brain. This enzyme has both calcium-independent phospholipase A(2) and transacylase activities. The discovery of this enzyme led us to propose a new pathway for ceramide metabolism in which the sn-2-acyl group of either phosphatidylethanolamine or phosphatidylcholine is ... More
Detection of cytosine methylation in RNA using bisulfite sequencing.
AuthorsPollex T, Hanna K, Schaefer M
JournalCold Spring Harb Protoc
PubMed ID20889702
'Post-transcriptional RNA modifications are a characteristic feature of noncoding RNAs and have been described for ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), and various other small RNAs. However, the biological function of most of these modifications remains uncharacterized. Cytosine-5 methylation (5mC) has been detected in abundant and long-lived RNA molecules such ... More
Unique DNA methylome profiles in CpG island methylator phenotype colon cancers.
AuthorsXu Y, Hu B, Choi AJ, Gopalan B, Lee BH, Kalady MF, Church JM, Ting AH
JournalGenome Res
PubMed ID21990380
'A subset of colorectal cancers was postulated to have the CpG island methylator phenotype (CIMP), a higher propensity for CpG island DNA methylation. The validity of CIMP, its molecular basis, and its prognostic value remain highly controversial. Using MBD-isolated genome sequencing, we mapped and compared genome-wide DNA methylation profiles of ... More
Enhanced autointegration in hyperstable simian immunodeficiency virus capsid mutants blocked after reverse transcription.
AuthorsTipper C, Sodroski J
JournalJ Virol
PubMed ID23345510
'After entering a host cell, retroviruses such as simian immunodeficiency virus (SIV) uncoat, disassembling the viral capsid. Rates of uncoating that are too high and too low can be detrimental to the efficiency of infection. Rapid uncoating typically leads to blocks in reverse transcription, but the basis for replication defects ... More
Thioredoxin 1 is responsible for antibody disulfide reduction in CHO cell culture.
AuthorsKoterba KL, Borgschulte T, Laird MW
JournalJ Biotechnol
PubMed ID22138638
During large-scale manufacturing of an IgG1 monoclonal antibody in Chinese hamster ovary (CHO) cells, reduction of the antibody's disulfide bonds was observed. We present evidence that mammalian thioredoxin 1 (TXN1) is the terminal enzyme responsible for this reduction event. We demonstrate a marked prevention of IgG1 disulfide bond reduction in ... More
Identification of the tRNA-dihydrouridine synthase family.
5,6-Dihydrouridine (D) is a modified base found abundantly in the D-loops of tRNA from Archaea, Bacteria, and Eukarya. D is thought to be formed post-transcriptionally by the reduction of uridines in tRNA transcripts. Despite its abundance, no enzymes that catalyze D-formation have been identified. Using comparative genomics and computational methods ... More
Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage.
Authors Matuschewski Kai; Ross Jessica; Brown Stuart M; Kaiser Karine; Nussenzweig Victor; Kappe Stefan H I;
JournalJ Biol Chem
PubMed ID12177071
Injection of Plasmodium salivary gland sporozoites into the vertebrate host by Anopheles mosquitoes initiates malaria infection. Sporozoites develop within oocysts in the mosquito midgut and then enter and mature in the salivary glands. Although morphologically similar, oocyst sporozoites and salivary gland sporozoites differ strikingly in their infectivity to the mammalian ... More
Resonance Raman and ligand binding studies of the oxygen-sensing signal transducer protein HemAT from Bacillus subtilis.
HemAT-Bs is a heme-containing signal transducer protein responsible for aerotaxis of Bacillus subtilis. The recombinant HemAT-Bs expressed in Escherichia coli was purified as the oxy form in which oxygen was bound to the ferrous heme. Oxygen binding and dissociation rate constants were determined to be k(on) = 32 microm(-1) s(-1) ... More
Cloning and characterization of ADAMTS-14, a novel ADAMTS displaying high homology with ADAMTS-2 and ADAMTS-3.
Authors Colige Alain; Vandenberghe Isabel; Thiry Marc; Lambert Charles A; Van Beeumen Jozef; Li Shi-Wu; Prockop Darwin J; Lapiere Charles M; Nusgens Betty V;
JournalJ Biol Chem
PubMed ID11741898
The processing of amino- and carboxyl-propeptides of fibrillar collagens is required to generate collagen monomers that correctly assemble into fibrils. Mutations in the ADAMTS2 gene, the aminopropeptidase of procollagen I and II, result in the accumulation of non-fully processed type I procollagen, causing human Ehlers-Danlos syndrome type VIIC and animal ... More
Characterization of two novel nuclear BTB/POZ domain zinc finger isoforms. Association with differentiation of hippocampal neurons, cerebellar granule cells, and macroglia.
Authors Mitchelmore Cathy; Kjaerulff Karen M; Pedersen Hans C; Nielsen Jakob V; Rasmussen Thomas E; Fisker Mads F; Finsen Bente; Pedersen Karen M; Jensen Niels A;
JournalJ Biol Chem
PubMed ID11744704
BTB/POZ (broad complex tramtrack bric-a-brac/poxvirus and zinc finger) zinc finger factors are a class of nuclear DNA-binding proteins involved in development, chromatin remodeling, and cancer. However, BTB/POZ domain zinc finger factors linked to development of the mammalian cerebral cortex, cerebellum, and macroglia have not been described previously. We report here ... More
Genome and transcriptome sequencing of lung cancers reveal diverse mutational and splicing events.
AuthorsLiu J, Lee W, Jiang Z, Chen Z, Jhunjhunwala S, Haverty PM, Gnad F, Guan Y, Gilbert HN, Stinson J, Klijn C, Guillory J, Bhatt D, Vartanian S, Walter K, Chan J, Holcomb T, Dijkgraaf P, Johnson S, Koeman J, Minna JD, Gazdar AF, Stern HM, Hoeflich KP, Wu TD, Settleman J, de Sauvage FJ, Gentleman RC, Neve RM, Stokoe D, Modrusan Z, Seshagiri S, Shames DS, Zhang Z
JournalGenome Res
PubMed ID23033341
Lung cancer is a highly heterogeneous disease in terms of both underlying genetic lesions and response to therapeutic treatments. We performed deep whole-genome sequencing and transcriptome sequencing on 19 lung cancer cell lines and three lung tumor/normal pairs. Overall, our data show that cell line models exhibit similar mutation spectra ... More
Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. One subfamily composed of l(2)35Aa is essentail in Drosophila.
Authors Schwientek Tilo; Bennett Eric P; Flores Carlos; Thacker John; Hollmann Martin; Reis Celso A; Behrens Jane; Mandel Ulla; Keck Birgit; Schäfer Mireille A; Haselmann Kim; Zubarev Roman; Roepstorff Peter; Burchell Joy M; Taylor-Papadimitriou Joyce; Hollingsworth Michael A; Clausen Henrik;
JournalJ Biol Chem
PubMed ID11925450
The completed fruit fly genome was found to contain up to 15 putative UDP-N-acetyl-alpha-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-transferase) genes. Phylogenetic analysis of the putative catalytic domains of the large GalNAc-transferase enzyme families of Drosophila melanogaster (13 available), Caenorhabditis elegans (9 genes), and mammals (12 genes) indicated that distinct subfamilies of orthologous genes ... More