Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells.
AuthorsEndo T; Ohta K; Haraguchi K; Onaya T;
JournalJ Biol Chem
PubMed ID7738021
Thyrotropin receptor (TSH-R) has been thought to be thyroid-specific, but, by Northern blot analysis, we found that rat adipose tissue expressed TSH-R mRNAs in amounts approaching those in the thyroid. To investigate the function of TSH-R from adipose tissue, we screened a rat fat cell lambda gt11 cDNA library for ... More
Modulation of mouse Paneth cell alpha-defensin secretion by mIKCa1, a Ca2+-activated, intermediate conductance potassium channel.
Authors Ayabe Tokiyoshi; Wulff Heike; Darmoul Dalila; Cahalan Michael D; Chandy K George; Ouellette Andre J;
JournalJ Biol Chem
PubMed ID11724775
'Paneth cells in small intestinal crypts secrete microbicidal alpha-defensins in response to bacteria and bacterial antigens (Ayabe, T., Satchell, D. P., Wilson, C. L., Parks, W. C., Selsted, M. E., and Ouellette, A. J. (2000) Nat. Immunol. 1, 113- 38). We now report that the Ca(2+)-activated K(+) channel mIKCa1 modulates ... More
Variant forms of alpha-fetoprotein transcripts expressed in human hematopoietic progenitors. Implications for their developmental potential towards endoderm.
Authors Kubota Hiroshi; Storms Robert W; Reid Lola M;
JournalJ Biol Chem
PubMed ID12006569
'Hematopoietic stem cells have been identified as multipotent cells that give rise to all adult hematopoietic lineages. Although the hematopoietic lineage is derived from the mesodermal germ layer in the embryo, recent data suggest that bone marrow cells with an antigenic profile consistent with that of hematopoietic stem cells can ... More
Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in Arabidopsis.
Authors Golldack Dortje; Popova Olga V; Dietz Karl-Josef;
JournalJ Biol Chem
PubMed ID11726650
'This study characterizes the expression and functional significance of the member of the matrix metalloproteinase (MMP) family At2-MMP from Arabidopsis. By transcript analysis, expression of At2-MMP was found in leaves and roots of juvenile Arabidopsis and leaves, roots, and inflorescences of mature flowering plants showing strong increase of transcript abundance ... More
Phosphorylation of the Carboxyl Terminus of Inner Centromere Protein (INCENP) by the Aurora B Kinase Stimulates Aurora B Kinase Activity.
Authors Bishop John D; Schumacher Jill M;
JournalJ Biol Chem
PubMed ID12048181
'How the events of mitosis are coordinated is not well understood. Intriguing mitotic regulators include the chromosomal passenger proteins. Loss of either of the passengers inner centromere protein (INCENP) or the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Furthermore, INCENP and Aurora B have identical ... More
Defective ureagenesis in mice carrying a liver-specific disruption of hepatocyte nuclear factor 4alpha (HNF4alpha ). HNF4alpha regulates ornithine transcarbamylase in vivo.
Authors Inoue Yusuke; Hayhurst Graham P; Inoue Junko; Mori Masataka; Gonzalez Frank J;
JournalJ Biol Chem
PubMed ID11994307
'Hepatocyte nuclear factor 4alpha (HNF4alpha) regulates the expression of many genes preferentially expressed in liver. HNF4alpha-null mice die during embryogenesis precluding the analysis of its function in the adult. To circumvent this problem, liver-specific HNF4alpha-null mice were produced. Mice lacking hepatic HNF4alpha expression exhibited increased serum ammonia and reduced serum ... More
Dual DNA binding specificity of ADD1/SREBP1 controlled by a single amino acid in the basic helix-loop-helix domain.
'Adipocyte determination- and differentiation-dependent factor 1 (ADD1), a member of the basic helix-loop-helix (bHLH) family of transcription factors, has been associated with both adipocyte differentiation and cholesterol homeostasis (in which case it has been termed SREBP1). Using PCR-amplified binding analysis, we demonstrate that ADD1/SREBP1 has dual DNA sequence specificity, binding ... More
Methylation-mediated silencing of TMS1/ASC is accompanied by histone hypoacetylation and CpG island-localized changes in chromatin architecture.
Authors Stimson Krista M; Vertino Paula M;
JournalJ Biol Chem
PubMed ID11733524
'Aberrant methylation of CpG-dense islands in the promoter regions of genes is an acquired epigenetic alteration associated with the silencing of tumor suppressor genes in human cancers. In a screen for endogenous targets of methylation-mediated gene silencing, we identified a novel CpG island-associated gene, TMS1, which is aberrantly methylated and ... More
Distribution of mutations in human thymidylate synthase yielding resistance to 5-fluorodeoxyuridine.
AuthorsKawate H, Landis DM, Loeb LA,
JournalJ Biol Chem
PubMed ID12147691
'Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and is the target of cancer chemotherapeutic agents (e.g. 5-fluorouracil). Here, we used error-prone PCR to mutagenize the full-length human TS cDNA and then selected mutants resistant to 5-fluorodeoxyuridine in a bacterial complementation system. We found that resistant mutants contained 1-5 ... More
Sequential mutations in the interleukin-3 (IL3)/granulocyte-macrophage colony-stimulating factor/IL5 receptor beta-subunit genes are necessary for the complete conversion to growth autonomy mediated by a truncated beta C subunit.
AuthorsHannemann J, Hara T, Kawai M, Miyajima A, Ostertag W, Stocking C
JournalMol Cell Biol
PubMed ID7739524
'An amino-terminally truncated beta C receptor (beta C-R) subunit of the interleukin-3 (IL3)/granulocyte-macrophage colony-stimulating factor/IL5 receptor complex mediates factor-independent and tumorigenic growth in two spontaneous mutants of a promyelocytic cell line. The constitutive activation of the JAK2 protein kinase in these mutants confirms that signaling occurs through the truncated receptor ... More
Isolation and expression of a cDNA clone encoding an Alternaria alternata Alt a 1 subunit.
'Alternaria alternata is recognized as an important source of fungal aeroallergens. Alt a 1, the major allergen of this mold, is a dimer of disulfide-linked subunits that migrate in SDS-PAGE under reducing conditions at apparent M(r)s of 14,500 and 16,000. IgE antibodies to this protein are present in the sera ... More
Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells.
Authors Tang Shaohua; Bhatia Bobby; Maldonado Carlos J; Yang Peiying; Newman Robert A; Liu Junwei; Chandra Dhyan; Traag Jeanine; Klein Russell D; Fischer Susan M; Chopra Dharam; Shen Jianjun; Zhau Haiyen E; Chung Leland W K; Tang Dean G;
JournalJ Biol Chem
PubMed ID11839751
'15-Lipoxygenase 2 (15-LOX2) is a recently cloned human lipoxygenase that shows tissue-restricted expression in prostate, lung, skin, and cornea. The protein level and enzymatic activity of 15-LOX2 have been shown to be down-regulated in prostate cancers compared with normal and benign prostate tissues. The biological function of 15-LOX2 and the ... More
Isoforms of cyclic nucleotide phosphodiesterase PDE3A in cardiac myocytes.
PDE3A cyclic nucleotide phosphodiesterases regulate cAMP- and cGMP-mediated intracellular signaling in cardiac myocytes. We used antibodies to different regions of PDE3A to demonstrate the presence of three PDE3A isoforms in these cells. These isoforms, whose apparent molecular weights are 136,000, 118,000, and 94,000 ( ... More
A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons.
Lack of appropriate tools and techniques to study fate and functional integration of newly generated neurons has so far hindered understanding of neurogenesis' relevance under physiological and pathological conditions. Current analyses are either dependent on mitotic labeling, for example BrdU-incorporation or retroviral infection, or on the detection of transient immature ... More
Virus-specific cytotoxic T-lymphocyte responses select for amino-acid variation in simian immunodeficiency virus Env and Nef.
AuthorsEvans DT, O'Connor DH, Jing P, Dzuris JL, Sidney J, da Silva J, Allen TM, Horton H, Venham JE, Rudersdorf RA,Vogel T, Pauza CD, Bontrop RE, DeMars R, Sette A, Hughes AL, Watkins DI
JournalNature Medicine
PubMed ID10545993
Cytotoxic T-lymphocyte (CTL) responses to human immunodeficiency virus arise early after infection, but ultimately fail to prevent progression to AIDS. Human immunodeficiency virus may evade the CTL response by accumulating amino-acid replacements within CTL epitopes. We studied 10 CTL epitopes during the course of simian immunodeficiency virus disease progression in ... More
Inhibition of Adipogenesis by Wnt Signaling
AuthorsSarah E. Ross, Nahid Hemati, Kenneth A. Longo, Christina N. Bennett, Peter C. Lucas, Robin L. Erickson, and Ormond A. MacDougald
JournalScience
PubMed ID10937998
Wnts are secreted signaling proteins that regulate developmental processes. Here we show that Wnt signaling, likely mediated by Wnt-10b, is a molecular switch that governs adipogenesis. Wnt signaling maintains preadipocytes in an undifferentiated state through inhibition of the adipogenic transcription factors CCAAT/enhancer binding protein (C/EBP ) and peroxisome ... More
Altered editing of serotonin 2C receptor pre-mRNA in the prefrontal cortex of depressed suicide victims.
AuthorsGurevich I, Tamir H, Arango V, Dwork AJ, Mann JJ, Schmauss C,
JournalNeuron
PubMed ID11988167
Five adenosines within the coding sequence of the serotonin 2C receptor (5-HT2C) pre-mRNA are converted to inosines by RNA editing (named A, B, C' (E), C, and D sites). In human prefrontal cortex (PFC), the most abundant 5-HT2C mRNA sequences result from editing at the A site, or from the ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
AuthorsGhanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
JournalJ Immunol
PubMed ID15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Occludin 1B, a Variant of the Tight Junction Protein Occludin
AuthorsZoia Muresan,* David L. Paul, and Daniel A. Goodenough*
JournalMol Cell Biol
PubMed ID10679019
Occludin and claudin are the major integral membrane components of themammalian tight junction. Although more than 11 distinct claudins have beenidentified, only 1 occludin transcript has been reported thus far. Therefore, wesearched by reverse transcription-PCR for occludin-related sequences inMadin-Darby canine kidney (MDCK) mRNA and identified a transcript encodingan alternatively spliced ... More
Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms.
A central question in lignin biosynthesis is how guaiacyl intermediates are hydroxylated and methylated to the syringyl monolignol in angiosperms. To address this question, we cloned cDNAs encoding a cytochrome P450 monooxygenase (LsM88) and a caffeate O-methyltransferase (COMT) from sweetgum (Liquidambar styraciflua) xylem. Mass spectrometry-based functional analysis of LsM88 in ... More
Overexpression of myotonic dystrophy kinase in BC3H1 cells induces the skeletal muscle phenotype.
AuthorsBush EW, Taft CS, Meixell GE, Perryman MB
JournalJ Biol Chem
PubMed ID8550617
Myotonic muscular dystrophy is an autosomal dominant defect that produces muscle wasting, myotonia, and cardiac conduction abnormalities. The myotonic dystrophy locus codes for a putative serine- threonine protein kinase of unknown function. We report that overexpression of human myotonic dystrophy protein kinase induces the expression of skeletal muscle-specific genes in ... More
Novel Isoform of Insulin Receptor Substrate p53/p58 Is Generated by Alternative Splicing in the CRIB/SH3-binding Region.
Authors Alvarez Carlos E; Sutcliffe J Gregor; Thomas Elizabeth A;
JournalJ Biol Chem
PubMed ID12006592
Insulin receptor substrate p53/p58 (IRSp53) is involved in cytoskeletal dynamics and is a candidate disease sensor in polyglutamine expansion neurodegeneration. It is widely expressed throughout the body, but its levels are dramatically elevated in forebrain regions. IRSp53 functions as a signal transducing adaptor between activated Rho family GTPases and their ... More
Cyclin L is an RS domain protein involved in pre-mRNA splicing.
Authors Dickinson Liliane A; Edgar Alasdair J; Ehley Jennifer; Gottesfeld Joel M;
JournalJ Biol Chem
PubMed ID11980906
We report the cDNA cloning and functional characterization of human cyclin L, a novel cyclin related to the C-type cyclins that are involved in regulation of RNA polymerase II (pol II) transcription. Cyclin L also contains a COOH-terminal dipeptide repeat of alternating arginines and serines, a hallmark of the SR ... More
Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos.
AuthorsDoyle J, Ellis SA, O'Gorman GM, Aparicio Donoso IM, Lonergan P, Fair T
JournalJ Reprod Immunol
PubMed ID19682752
The role of the Major Histocompatibility Complex class I (MHC-I) genes in human and mouse preimplantation embryo development has received considerable attention. In contrast, information concerning the role of these genes in bovine embryo development is limited. The objective of the current study was to characterize the expression pattern of ... More
Expression and localization of the Parkin co-regulated gene in mouse CNS suggests a role in ependymal cilia function.
AuthorsWilson GR, Tan JT, Brody KM, Taylor JM, Delatycki MB, Lockhart PJ
JournalNeurosci Lett
PubMed ID19463890
Parkin Co-Regulated Gene (PACRG) is a gene that shares a bi-directional promoter with the Parkinson's disease associated gene parkin. The functional role of PACRG is not well understood, although the gene has been associated with parkinsonian syndromes and more recently with eukaryotic cilia and flagella. We investigated the expression of ... More
Genomic analysis of the carboxylesterases: identification and classification of novel forms.
AuthorsWilliams ET, Wang H, Wrighton SA, Qian YW, Perkins EJ
JournalMol Phylogenet Evol
PubMed ID20510380
Large species differences in the expression of carboxylesterases (CE) have been described, but the interrelationships of CEs across species are not well characterized. In the current analyses, sequences with genomic structures similar to human CEs were found in piscine, avian, and mammalian genomes. Analyses of these genes suggest that four ... More
Antidiabetic Effect of a Prodrug of Cysteine, L-2-Oxothiazolidine-4-carboxylic Acid, through CD38 Dimerization and Internalization.
Authors Han Myung-Kwan; Kim Se-Jin; Park Young-Ran; Shin Young-Mi; Park Hyun-Jung; Park Kum-Jae; Park Kwang-Hyun; Kim Hyun-Kag; Jang Seon-Il; An Nyeon-Hyoung; Kim Uh-Hyun;
JournalJ Biol Chem
PubMed ID11679582
CD38 is a bifunctional enzyme synthesizing (ADP-ribosyl cyclase) and degrading (cyclic ADP-ribose (cADPR) hydrolase) cADPR, a potent Ca(2+) mobilizer from intracellular pools. CD38 internalization has been proposed as a mechanism by which the ectoenzyme produced intracellular cADPR, and thiol compounds have been shown to induce the internalization of CD38. Here, ... More
Cloning of expressed sequence tags (ESTs) representing putative epileptogenesis-related genes and the localization of their expression in the normal brain.
AuthorsStefaniuk M, Lukasiuk K
JournalNeurosci Lett
PubMed ID20655365
The goal of this study was to clone and sequence selected expressed sequence tags (ESTs) which mRNA is upregulated in a rat model of epilepsy based on our previous work [17] and to determine the localization of their mRNA expression in the normal brain. Six ESTs that had not been ... More
The ADP-ribosylating mosquitocidal toxin from Bacillus sphaericus: proteolytic activation, enzyme activity, and cytotoxic effects.
Authors Schirmer Jörg; Just Ingo; Aktories Klaus;
JournalJ Biol Chem
PubMed ID11812773
The mosquitocidal toxin (MTX) from Bacillus sphaericus SSII-1 is a approximately 97-kDa protein sharing sequence homology within the N terminus with the catalytic domains of various bacterial ADP-ribosyltransferases. Here we studied the proteolytic activation of the ADP-ribosyltransferase activity of MTX. Chymotrypsin treatment of the 97-kDa MTX holotoxin (MTX(30-870)) results in ... More
Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues.
Thiamine triphosphate (ThTP) is found at low concentrations in most animal tissues, and recent data suggest that it may act as a phosphate donor for the phosphorylation of some proteins. In the mammalian brain, ThTP synthesis is rapid, but its steady-state concentration remains low, presumably because of rapid hydrolysis. In ... More
Human alpha-synuclein-harboring familial Parkinson's disease-linked Ala-53 --> Thr mutation causes neurodegenerative disease with alpha-synuclein aggregation in transgenic mice.
Authors Lee Michael K; Stirling Wanda; Xu Yanqun; Xu Xueying; Qui Dike; Mandir Allen S; Dawson Ted M; Copeland Neal G; Jenkins Nancy A; Price Don L;
JournalProc Natl Acad Sci U S A
PubMed ID12084935
Mutations in alpha-synuclein (alpha-Syn) cause Parkinson's disease (PD) in a small number of pedigrees with familial PD. Moreover, alpha-Syn accumulates as a major component of Lewy bodies and Lewy neurites, intraneuronal inclusions that are neuropathological hallmarks of PD. To better understand the pathogenic relationship between alterations in the biology of ... More
Regulation of Wnt signaling during adipogenesis.
AuthorsBennett CN, Ross SE, Longo KA, Bajnok L, Hemati N, Johnson KW, Harrison SD, MacDougald OA.
JournalJ Biol Chem
PubMed ID12055200
We have identified Wnt10b as a potent inhibitor of adipogenesis that must be suppressed for preadipocytes to differentiate in vitro. Here, we demonstrate that a specific inhibitor of glycogen synthase kinase 3, CHIR 99021, mimics Wnt signaling in preadipocytes. CHIR 99021 stabilizes free cytosolic beta-catenin and inhibits adipogenesis by blocking ... More
ATPase activity of the terminase subunit pUL56 of human cytomegalovirus.
Authors Hwang Jae-Seon; Bogner Elke;
JournalJ Biol Chem
PubMed ID11744697
Herpesviral DNA packaging is a complex process resulting in unit-length genomes packed into preformed procapsids. This process is believed to be mediated by two packaging proteins, the terminase subunits. In the case of double-stranded DNA bacteriophages, the translocation of DNA was shown to be an energy-dependent process associated with an ... More
Analysis of the Complexity of Protein Kinases within the Phloem Sieve Tube System. CHARACTERIZATION OF CUCURBITA MAXIMA CALMODULIN-LIKE DOMAIN PROTEIN KINASE 1.
Authors Yoo Byung-Chun; Lee Jung-Youn; Lucas William J;
JournalJ Biol Chem
PubMed ID11847230
In angiosperms, functional, mature sieve elements lack nuclei, vacuoles, ribosomes, and most of the endomembrane network. In this study, the complexity, number, and nature of protein kinases within the phloem sap of Cucurbita maxima were investigated to test the hypothesis that the enucleate sieve tube system utilizes a simplified signal ... More
In vivo footprinting of the human 11beta-hydroxysteroid dehydrogenase type 2 promoter: evidence for cell-specific regulation by Sp1 and Sp3.
Authors Nawrocki Andrea R; Goldring Christopher E; Kostadinova Radina M; Frey Felix J; Frey Brigitte M;
JournalJ Biol Chem
PubMed ID11850421
11beta-Hydroxysteroid dehydrogenase type 2 is selectively expressed in aldosterone target tissues, where it confers aldosterone selectivity for the mineralocorticoid receptor by inactivating 11beta-hydroxyglucocorticoids with a high affinity for the mineralocorticoid receptor. The present investigation aimed to elucidate the mechanisms accounting for the rigorous control of the HSD11B2 gene in humans. ... More
Molecular cloning and characterization of STAMP1, a highly prostate-specific six transmembrane protein that is overexpressed in prostate cancer.
Authors Korkmaz Kemal S; Elbi Cem; Korkmaz Ceren G; Loda Massimo; Hager Gordon L; Saatcioglu Fahri;
JournalJ Biol Chem
PubMed ID12095985
We have identified a novel gene, six transmembrane protein of prostate 1 (STAMP1), which is largely specific to prostate for expression and is predicted to code for a 490-amino acid six transmembrane protein. Using a form of STAMP1 labeled with green fluorescent protein in quantitative time-lapse and immunofluorescence confocal microscopy, ... More
Differential effects of a centrally acting fatty acid synthase inhibitor in lean and obese mice.
Authors Kumar Monica V; Shimokawa Teruhiko; Nagy Tim R; Lane M Daniel;
JournalProc Natl Acad Sci U S A
PubMed ID11854492
C75 is a potent inhibitor of fatty acid synthase that acts centrally to reduce food intake and body weight in mice; a single dose causes a rapid (>90%) decrease of food intake. These effects are associated with inhibition of fasting-induced up-regulation and down-regulation, respectively, of the expression of orexigenic (NPY ... More
Functional knockout of the adenosine 5'-phosphosulfate reductase gene in Physcomitrella patens revives an old route of sulfate assimilation.
The reduction of adenosine 5'-phosphosulfate (APS) to sulfite catalyzed by adenosine 5'-phosphosulfate reductase is considered to be the key step of sulfate assimilation in higher plants. However, analogous to enteric bacteria, an alternative pathway of sulfate reduction via phosphoadenosine 5'-phosphosulfate (PAPS) was proposed. To date, the presence of the corresponding ... More
Biosynthesis and cellular trafficking of the convertase SKI-1/S1P: ectodomain shedding requires SKI-1 activity.
Subtilisin kexin isozyme-1 (SKI-1)/site 1 protease is a mammalian subtilase composed of distinct functional domains. Among the major substrates of SKI-1 are the sterol regulatory element-binding proteins, regulating cholesterol and fatty acid homeostasis. Other substrates include the stress response factor activating transcription factor-6, the brain-derived neurotrophic factor, and the surface ... More
Hypoxia-inducible factor-1-mediated expression of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) gene. Its possible role in the Warburg effect.
Authors Minchenko Alexander; Leshchinsky Irene; Opentanova Irina; Sang Nianli; Srinivas Vickram; Armstead Valerie; Caro Jaime;
JournalJ Biol Chem
PubMed ID11744734
One of the key mediators of the hypoxic response in animal cells is the hypoxia-inducible transcription factor-1 (HIF-1) complex, in which the alpha-subunit is highly susceptible to oxygen-dependent degradation. The hypoxic response is manifested in many pathophysiological processes such as tumor growth and metastasis. During hypoxia, cells shift to a ... More
The N-terminal coiled coil domain of the cytohesin/ARNO family of guanine nucleotide exchange factors interacts with the scaffolding protein CASP.
Authors Mansour Marc; Lee Stella Y; Pohajdak Bill;
JournalJ Biol Chem
PubMed ID12052827
Cytohesin is a guanine nucleotide exchange factor that regulates members of the ADP-ribosylation factor (ARF) family of small GTPases. All of the members of the cytohesin family (including ARNO, ARNO3, and the newly characterized cytohesin-4) have a similar domain distribution consisting of a Sec7 homology domain, a pleckstrin homology domain, ... More
Identification of amino acid residues critical for LD78beta, a variant of human macrophage inflammatory protein-1alpha, binding to CCR5 and inhibition of R5 human immunodeficiency virus type 1 replication.
In an attempt to determine which amino acid(s) of LD78beta, a variant of human macrophage inflammatory protein-1alpha, plays a critical role in the interaction with CCR5, we generated six LD78beta variants with an amino acid substituted to Ala at the NH(2) terminus of LD78beta. There was no significant difference in ... More
Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role in canalicular bile secretion.
Authors Huebert Robert C; Splinter Patrick L; Garcia Fabiana; Marinelli Raul A; LaRusso Nicholas F;
JournalJ Biol Chem
PubMed ID11932260
Although bile formation requires that large volumes of water be rapidly transported across liver epithelia, including hepatocytes, the molecular mechanisms by which water is secreted into bile are obscure. The aquaporins are a family of 10 channel-forming, integral membrane proteins of approximately 28 kDa numbered 0-9 that allow water to ... More
High glucose stimulates synthesis of fibronectin via a novel protein kinase C, Rap1b, and B-Raf signaling pathway.
Authors Lin Sun; Sahai Atul; Chugh Sumant S; Pan Xiaomin; Wallner Elisabeth I; Danesh Farhad R; Lomasney Jon W; Kanwar Yashpal S;
JournalJ Biol Chem
PubMed ID12196513
The molecular mechanism(s) by which high glucose induces fibronectin expression via G-protein activation in the kidney are largely unknown. This investigation describes the effect of high glucose (HG) on a small GTP-binding protein, Rap1b, expression and activation, and the relevance of protein kinase C (PKC) and Raf pathways in fibronectin ... More
Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator.
Authors Zhang Hui; Peters Kathryn W; Sun Fei; Marino Christopher R; Lang Jochen; Burgoyne Robert D; Frizzell Raymond A;
JournalJ Biol Chem
PubMed ID12039948
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel whose phosphorylation regulates both channel gating and its trafficking at the plasma membrane. Cysteine string proteins (Csps) are J-domain-containing, membrane-associated proteins that have been functionally implicated in regulated exocytosis. Therefore, we evaluated the possibility that Csp is involved ... More
Function assignment to conserved residues in mammalian alkaline phosphatases.
We have probed the structural/functional relationship of key residues in human placental alkaline phosphatase (PLAP) and compared their properties with those of the corresponding residues in Escherichia coli alkaline phosphatase (ECAP). Mutations were introduced in wild-type PLAP, i.e. [E429]PLAP, and in some instances also in [G429]PLAP, which displays properties characteristic ... More
Generation of the mu opioid receptor (MOR-1) protein by three new splice variants of the Oprm gene.
Authors Pan Y X; Xu J; Mahurter L; Bolan E; Xu M; Pasternak G W;
JournalProc Natl Acad Sci U S A
PubMed ID11717463
Using 5' RACE, we have isolated four additional exons of the mu opioid receptor gene (Oprm), resulting in a gene spanning over 250 kb. The four new exons are contained within eight additional splice variants containing exon 11 at the 5' terminus. Exon 11, which is under the control of ... More
Cloning and characterization of human Siglec-11. A recently evolved signaling that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia.
Authors Angata Takashi; Kerr Sheena C; Greaves David R; Varki Nissi M; Crocker Paul R; Varki Ajit;
JournalJ Biol Chem
PubMed ID11986327
Siglecs are sialic acid-recognizing animal lectins of the immunoglobulin superfamily. We have cloned and characterized a novel human molecule, Siglec-11, that belongs to the subgroup of CD33/Siglec-3-related Siglecs. As with others in this subgroup, the cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and ... More
Calmodulin modulates the delay period between release of calcium from internal stores and activation of calcium influx via endogenous TRP1 channels.
Authors Vaca Luis; Sampieri Alicia;
JournalJ Biol Chem
PubMed ID12196518
In the present study we have explored the role of calmodulin (CaM) and inositol 1,4,5-trisphosphate receptor (IP(3)R) in the communication process activated after the release of calcium from the endoplasmic reticulum (ER) and the activation of calcium influx via endogenous TRP1 channels from Chinese hamster ovary cells. Experiments using combined ... More