Invitrogen™

TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

Catalog number: K460040
Invitrogen™

TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent E. coli cells

Catalog number: K460040
Catalog Number
K460040
also known as K4600-40
Unit Size
50 reactions
Price (USD)
Price: 1,290.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 reactions
Price (USD)
Price: 382.00
Your price:
Availability
-
Quantity
Catalog Number
K460001
also known as K4600-01
Unit Size
25 reactions
Price (USD)
Price: 710.00
Online price: 681.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K460040
also known as K4600-40
50 reactions
Price: 1,290.00
Your price:
-
K4600J1010 reactions
Price: 382.00
Your price:
-
K460001
also known as K4600-01
25 reactions
Price: 710.00
Online price: 681.65
Your price:
-
Product Overview
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TOPO™ TA Cloning™ Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the most reliable cloning method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.

Convenient features of this TOPO™ TA Cloning™ Dual Promoter kit include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Kit Options: TOPO™ TA Cloning™ Dual Promoter Kits
TOPO™ TA Cloning™ Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:
• General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
• High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40)
• General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
• Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20)
• Repressor/induction needs: TOP10F’ cells (Cat. Nos. K4650-01, K4650-40)
• Provide your own cells (Cat. Nos. 451641, 450641)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
TOP10
Cell Type
Chemically Competent
Cloning Method
TOPO™-TA
Format
Kit
Product Line
One Shot™
Product Type
Cloning Kit
Promoter
T7, SP6
Quantity
50 Reactions
Vector
TOPO-TA Cloning Vectors

Contents & Storage

Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.

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