TOPO® TA Cloning® Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO® TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable cloning method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.
Convenient features of this TOPO® TA Cloning® Dual Promoter kit include: • 3´-T overhangs for direct ligation of Taq-amplified PCR products • T7 and SP6 promoters for efficient in vitro transcription • M13 forward and reverse primer sites for sequencing • 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning • Kanamycin and ampicillin resistance for your choice of selection in E. coli • Easy blue/white colony screening for selection of recombinants • Includes OneShot® TOP10 Competent cells for added convenience and highest cloning efficiencies
Kit Options: TOPO® TA Cloning® Dual Promoter Kits TOPO® TA Cloning® Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs: • General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40) • High-efficiency cloning: TOP10 Electrocomp™ cells (Cat. Nos. K4660-01, K4660-40) • General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40) • Fast growth: Mach1™ -T1R chemically competent E. coli (Cat. No. K4610-20) • Repressor/induction needs: TOP10F’ cells (Cat. Nos. K4650-01, K4650-40) • Provide your own cells (Cat. Nos. 451641, 450641)
For Research Use Only. Not for use in diagnostic procedures.
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Kanamycin (KanR)
Bacterial or Yeast Strain:
Contents & storage
Box 1: • Topoisomerase I-activated pCR™II-TOPO® vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot® Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid.