Notice: This product will be discontinued on May 1, 2017. For superior performance, we recommend the TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells.

TOPO® XL PCR Cloning Kit, with One Shot® TOP10 Chemically Competent E. coli

Catalog number: K475010

Invitrogen™  Related applications: PCR Cloning

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The TOPO® XL PCR Cloning Kit combines a unique vector and novel protocol for easy, efficient cloning of long (3-10 kb) PCR products. The kit uses the linearized and topoisomerase I-activated pCR™-XL-TOPO® vector. Topoisomerase I activation allows PCR products to be ligated in just 5 minutes on your bench top and typically results in >80% recombinants. The pCR™-XL-TOPO® vector includes:

ccdB gene for positive selection
EcoR I and Nsi I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
• T7 promoter/priming site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing

Novel Protocol
The TOPO® XL PCR Cloning Kit protocol includes S.N.A.P.™ gel purification which employs crystal violet for improved efficiency of long PCR cloning. This eliminates the need for ethidium bromide and exposure to UV light, which can damage long PCR products and reduce the number of recombinants.
For Research Use Only. Not for use in diagnostic procedures.


Vector Type: TOPO-TA Cloning Vectors
Cloning Method: TOPO®-TA
Bacterial or Yeast Strain: TOP10
Product Line: One Shot®, TA Cloning®, TOPO®
Product Size: 10 reactions

Contents & storage

Each TOPO® XL PCR Cloning Kit includes linearized and topoisomerase I-activated pCR™-XL-TOPO® vector, dNTPs, control template and primers, M13 forward and reverse primers, sodium iodide solution, binding buffer, 4X final wash, S.N.A.P.™ purification columns and collection vials, 6X crystal violet loading buffer, 2 mg/ml crystal violet solution, One Shot® Electrocomp™ or Chemically Competent E. coli, S.O.C .medium, and pUC19 supercoiled control plasmid. Store One Shot® E. coli at -80°C. Store gel purification columns and reagents at room temperature. Store the remaining components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols

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