NT-GFP Fusion TOPO® Expression Kit

Catalog number: K481001

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

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The GFP Fusion TOPO® TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO® or pcDNA3.1/CT-GFP-TOPO® vectors (Figure 1). Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells (Figure 2). Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.
For Research Use Only. Not for use in diagnostic procedures.



Promoter: CMV
Key Function: Reporter Assays
Protein Tag or Fusion: GFP (Cycle 3)
Reporter: GFP (Cycle 3)
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors
Product Size: 20 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

GFP Fusion TOPO® Expression Kits contain two boxes. The GFP Fusion TOPO® TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot® box at -80°C. Kits are guaranteed stable for 6 months when properly stored.


Manuals & protocols

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