CT-GFP Fusion TOPO® Expression Kit

Catalog number: K482001

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Our CT GFP-fusion TOPO® Expression kit provides pcDNA™3.1⁄CT-GFP-TOPO® vector for highly efficient, 5-minute cloning that fuses the green fluorescent protein (GFP) to the C-terminal end your Taq polymerase-amplified gene product. The pcDNA3.1 vector delivers high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Reporter Assays
Protein Tag or Fusion: GFP (Cycle 3)
Reporter: GFP (Cycle 3)
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors
Constitutive or Inducible System: Constitutive
Product Size: 20 reactions

Contents & storage

CT-GFP Fusion Expression kit contain two boxes: 1. TOPO® cloning box with pcDNA™3.1⁄CT-GFP TOPO® cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored

Documents

Manuals & protocols

Recommended products