pGlow TOPO™ TA Expression Kit

Catalog number:  K483001

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

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The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.
For Research Use Only. Not for use in diagnostic procedures.



Promoter: None (Promoterless)
Key Function: Clone your own Promoter, Reporter Assays
Protein Tag or Fusion: Untagged
Reporter: GFP (Cycle 3)
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors, pGlow
Product Line: TOPO®
Product Size: 20 reactions

Contents & storage

pGlow TOPO® TA Expression Kits contains two boxes. The TOPO® TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO® vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot® box contains One Shot® TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.


Manuals & protocols