pBlue TOPO® TA Expression Kit

Catalog number: K483101

Invitrogen™  Related applications: Protein Assays and Analysis | Protein Expression

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The pBlue TOPO® TA Expression Kit uses β-galactosidase expression to assay a wide range of transcriptional activity from promoter fragments. Assays for β-galactosidase are easy to perform and are quantitative at very low levels of expression. The pBlue TOPO® TA Expression Kit is ideal for functional analysis of promoters with low transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures.



Promoter: None (Promoterless)
Key Function: Clone your own Promoter, Reporter Assays
Protein Tag or Fusion: Untagged
Reporter: Beta-Gal (lacZ)
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: TOPO-TA Vectors, pBlue
Product Size: 20 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

pBlue TOPO® TA Expression Kits contains two boxes. The TOPO® TA Cloning box contains linearized and topoisomerase I-activated pBlue-TOPO® vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers. This box should be stored at -20°C. The One Shot® box contains One Shot® TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.


Manuals & protocols

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