ViraPower™ Adenoviral Promoterless Gateway® Expression Kit

Catalog number: K494000

Invitrogen™  Related applications: Antibody & Cell Culture Production | Protein Expression | Viral Vaccine Production

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Description

The ViraPower™ Adenoviral Promoterless Gateway® Expression Kit combines Invitrogen’s ViraPower™ Adenoviral and Gateway® technologies to facilitate easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The pAd⁄PL-DEST™ vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway® Technology for efficient and rapid cloning
&bull Promoterless vector that allows gene of interest to be controlled by a promoter of choice
&bull Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
&bull Ampicillin selection marker

Kit includes
• pAd⁄PL-DEST™ Gateway® Vector (6 µg. Cat. No. V493-20)
• 293A Cell Line (3 x 106 cells, frozen. Cat. No. R705-07)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: None (Promoterless)
Key Function: Viral Expression, Clone your own Promoter
Protein Tag or Fusion: Untagged
Delivery Method: Adenoviral
Cloning Method: Gateway®
Vector Type: pAd
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

• pAd/PL-DEST Gateway® Vector (Store at -20°C)
• 293A Cell Line (Store in liquid nitrogen)

Documents

Manuals & protocols