The ViraPower™ Adenoviral Promoterless Gateway™ Expression Kit combines Invitrogen’s ViraPower™ Adenoviral and Gateway™ technologies to facilitate easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The pAd⁄PL-DEST™ vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.
Advantages • High efficiency and rapid recombination cloning • Produces high titer adenoviral stocks • Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo • Allows gene of interest to be controlled by a promoter of choice • Produces replication-incompetent virus for enhanced biosafety of the system • Amenable for use in high-throughput applications
Key Features • Gateway™ Technology for efficient and rapid cloning • Promoterless vector that allows gene of interest to be controlled by a promoter of choice • Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions • Ampicillin selection marker
Kit includes • pAd⁄PL-DEST™ Gateway™ Vector (6 μg. Cat. No. V493-20) • 293A Cell Line (3 x 106 cells, frozen. Cat. No. R705-07)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Adenoviral
Promoter
None (Promoterless)
Product Type
Adenoviral Expression Kit
Protein Tag
Untagged
Cloning Method
Gateway
Vector
pAd
Product Line
Gateway™, ViraPower™
For Use With (Application)
Clone your own Promoter, Viral Expression
Contents & Storage
• pAd/PL-DEST Gateway™ Vector (Store at -20°C) • 293A Cell Line (Store in liquid nitrogen)