Invitrogen™
ViraPower™ Lentiviral Gateway™ Expression Kit
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Description
The ViraPower™ Lentiviral Gateway® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-DEST™ vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells
Key Features
• Flexible and versatile Gateway® recombination cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection
Kit includes
• pLenti6⁄V5-DEST™ vector
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R70007)
• One Shot® Stbl3™ Chemically Competent E. coli (C7373-03)
Related SKUs
• pLenti6⁄V5™ Directional TOPO® Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO® Expression Kit (K4950-00)
• ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (K5330-00)
• ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit (K5310-00)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Promoter: | CMV |
|---|---|
| Key Function: | Viral Expression |
| Protein Tag or Fusion: | V5 Epitope Tag |
| Delivery Method: | Lentiviral |
| Cloning Method: | Gateway® |
| Selection Agent (Eukaryotic): | Blasticidin |
| Vector Type: | pLenti |
| Constitutive or Inducible System: | Constitutive |
| Product Line: | Gateway®, ViraPower™ |
| Product Size: | 1 kit |
Contents & storage
pLenti6⁄V5-DEST™ Gateway® Vector Kit
pLenti6⁄V5™ vectors: -20°C
One Shot® Stbl3™ Chemically Competent E. coli: -80°C
ViraPower™ Bsd Lentiviral Support Kit
ViraPower™ Packaging Mix –20°C
Lipofectamine® 2000 +4°C (DO NOT freeze)
Blasticidin: -20°C
293FT Cell Line: liquid nitrogen
pLenti6⁄V5™ vectors: -20°C
One Shot® Stbl3™ Chemically Competent E. coli: -80°C
ViraPower™ Bsd Lentiviral Support Kit
ViraPower™ Packaging Mix –20°C
Lipofectamine® 2000 +4°C (DO NOT freeze)
Blasticidin: -20°C
293FT Cell Line: liquid nitrogen
