ViraPower™ Lentiviral Gateway® Expression Kit

Catalog number: K496000

Invitrogen™  Related applications: Protein Expression

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Description

The ViraPower™ Lentiviral Gateway® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. It combines Invitrogen’s ViraPower™ Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄V5-DEST™ vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Flexible and versatile Gateway® recombination cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti6⁄V5-DEST™ vector
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R70007)
• One Shot® Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄V5™ Directional TOPO® Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO® Expression Kit (K4950-00)
• ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (K5330-00)
• ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit (K5310-00)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

pLenti6⁄V5-DEST™ Gateway® Vector Kit
pLenti6⁄V5™ vectors: -20°C
One Shot® Stbl3™ Chemically Competent E. coli: -80°C

ViraPower™ Bsd Lentiviral Support Kit
ViraPower™ Packaging Mix –20°C
Lipofectamine® 2000 +4°C (DO NOT freeze)
Blasticidin: -20°C

293FT Cell Line: liquid nitrogen

Documents

Manuals & protocols