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Invitrogen™

ViraPower™ Lentiviral Packaging Mix

Catalog number: K497500
Invitrogen™

ViraPower™ Lentiviral Packaging Mix

Catalog number: K497500
Catalog Number
K497500
Unit Size
60 rxns
Price (USD)
Price: 3,070.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K49750060 rxns
Price: 3,070.00
Your Price:
-
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ViraPower™ Lentiviral Packaging Mix contains an optimized mixture of the three packaging plasmids, pLP1, pLP2, and pLP/VSVG. These plasmids supply the helper functions as well as structural and replication proteins in trans required to produce a recombinant lentivirus, containing your gene of interest, using our ViraPower™ Lentiviral Expression System.

The ViraPower™ Lentiviral Expression System
The ViraPower™ Lentiviral Expression System allows creation of a replication-incompetent, HIV-1–based lentivirus that is used to deliver and express your gene of interest in either dividing or non-dividing mammalian cells. The major components of the system include:

• An expression plasmid containing the gene of interest under the control of a choice of promoters, and elements that allow packaging of the construct into virions
• An optimized mix of the three packaging plasmids (pLP1, pLP2, and pLP/VSVG) that supply the structural and replication proteins in trans that are required to produce the lentivirus
• The 293FT cell line, which allows production of lentivirus following cotransfection of the expression plasmid and the plasmids in the packaging mix
• Control expression plasmid to optimize virus production and cell transduction, containing either the lacZ gene—which when packaged into virions and transduced into a mammalian cell line, expresses β-galactosidase (included with each expression vector), or the Emerald Green Fluorescent Protein (EmGFP) gene—which when packaged into virions and transduced into a mammalian cell line, expresses EmGFP.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Constitutive
Delivery Type
Lentiviral
Promoter
RSV, CMV
Product Type
Lentiviral Packaging Mix
Selection Agent (Eukaryotic)
Blasticidin
Protein Tag
V5 Epitope Tag
Cloning Method
TOPO™-TA, Gateway™
Vector
pLP
Product Line
ViraPower™
For Use With (Application)
Viral Expression

Contents & Storage

Three tubes, each containing 195 µg lyophilized mix. The packaging mix is enough for 60 transfection reactions in 10 cm tissue culture dishes. Store at -20°C.