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ViraPower™ Lentiviral Packaging Mix

Catalog number: K497500

Invitrogen™  Related applications: Protein Expression

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Description

ViraPower™ Lentiviral Packaging Mix contains an optimized mixture of the three packaging plasmids, pLP1, pLP2, and pLP/VSVG. These plasmids supply the helper functions as well as structural and replication proteins in trans required to produce a recombinant lentivirus, containing your gene of interest, using our ViraPower™ Lentiviral Expression System.

The ViraPower™ Lentiviral Expression System
The ViraPower™ Lentiviral Expression System allows creation of a replication-incompetent, HIV-1–based lentivirus that is used to deliver and express your gene of interest in either dividing or non-dividing mammalian cells. The major components of the system include:

• An expression plasmid containing the gene of interest under the control of a choice of promoters, and elements that allow packaging of the construct into virions
• An optimized mix of the three packaging plasmids (pLP1, pLP2, and pLP/VSVG) that supply the structural and replication proteins in trans that are required to produce the lentivirus
• The 293FT cell line, which allows production of lentivirus following cotransfection of the expression plasmid and the plasmids in the packaging mix
• Control expression plasmid to optimize virus production and cell transduction, containing either the lacZ gene—which when packaged into virions and transduced into a mammalian cell line, expresses β-galactosidase (included with each expression vector), or the Emerald Green Fluorescent Protein (EmGFP) gene—which when packaged into virions and transduced into a mammalian cell line, expresses EmGFP.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV, RSV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®, TOPO®-TA
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLP
Product Size: 60 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

Three tubes, each containing 195 µg lyophilized mix. The packaging mix is enough for 60 transfection reactions in 10 cm tissue culture dishes. Store at -20°C.

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