ViraPower™ Zeo Lentiviral Support Kit

Catalog number: K498500

Invitrogen™  Related applications: Protein Expression

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Description

The ViraPower™ Zeo Lentiviral Support Kit contains the necessary components to transfect 293FT cells to generate viral particles containing the target gene. The kit contains the ViraPower™ Lentiviral Packaging Mix, Lipofectamine® 2000 Reagent and Zeocin™ and is designed to be used with a ViraPower™ Lentiviral expression vector that has the Zeocin™ marker for stable selection. The kit is compatible with all of our ViraPower™ Lentiviral vectors, including the ViraPower™ HiPerform™ Lentiviral vectors.

Kit includes
• ViraPower™ Lentiviral Packaging Mix
• Lipofectamine® 2000 (Cat # 11668027)
• Zeocin™ solution

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV, RSV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLP
Product Size: 20 reactions
Constitutive or Inducible System: Constitutive

Contents & storage

Contents
The ViraPower™ Zeo Lentiviral Support Kit includes the following components:
• 195 µg of ViraPower™ Packaging Mix (Contains 195 µL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1 µg⁄µL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine® 2000 (Cat # 11668027)
• 125 mg of Zeocin™ (1.25 ml of a 100mg⁄ml solution)

Storage
• ViraPower™ Packaging Mix: -20°C
• Lipofectamine® 2000: 4°C (do not freeze)
• Zeocin™: -20°C, protected from light

Documents

Manuals & protocols