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Invitrogen™
ViraPower™ UbC Lentiviral Gateway™ Expression Kit
The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K499000 | 1 kit |
카탈로그 번호 K499000
제품 가격(KRW)
3,697,000
Each
수량:
1 kit
제품 가격(KRW)
3,697,000
Each
The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen™’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄UbC⁄V5-DEST™ vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V499-10)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V499-10)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Lentiviral
용도(애플리케이션)Viral Expression
제품 유형Lentiviral Expression Kit
수량1 kit
선택 제제(진핵)Blasticidin
벡터pLenti
클로닝 방법Gateway
제품라인Gateway, ViraPower
프로모터UbC
단백질 태그V5 Epitope Tag
Unit SizeEach
구성 및 보관
Vector kit (Boxes 1 and 2)
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C
Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C
Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen