ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit

Catalog number: K531000

Invitrogen™  Related applications: Protein Expression

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The ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Typically provides 5 minute, high-efficiency TOPO® PCR cloning in three simple steps

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
•Blasticidin selection

Kit Includes
• pLenti6.3⁄V5-TOPO® TA Cloning® Kit (K5315-20)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Blasticidin
Vector Type: TOPO-TA Vectors, pLenti
Product Size: 1 kit
Special Elements: WPRE, cPPT
Constitutive or Inducible System: Constitutive

Contents & storage

TOPO® TA Cloning Reagents (Box 1)
• pLenti6.3⁄V5-TOPO® (5-10 ng⁄µL) 20 µL, -20°C
• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄µL) 20 µL, -20°C
• 10X PCR Buffer 100 µL, -20°C
• l dNTP Mix 10 µL, -20°C
• Salt Solution 50 µL, -20°C
• CMV Forward Primer (100 ng⁄µL) 20 µL, -20°C
• V5 (C-term) Reverse Primer (100 ng⁄µL) 20 µL, -20°C
• Control PCR Template (50 ng⁄µL) 10 µL, -20°C
• Control PCR Primers (100 ng⁄µL) 10 µL each, -20°C
• Sterile Water 1 mL

One Shot® Stbl3™ Chemically Competent E. coli (Box 2)
• Stbl3™ Cells 21 x 50 µL, -80°C
• pUC19 control DNA (10 pg⁄µL) 50 µL, -80°C
• S.O.C. Medium 6 mL, -80°C

Support kit
• ViraPower™ Packaging Mix 195 µg, -20°C
• Lipofectamine® 2000 0.75 mL, +4°C
• Blasticidin 50 mg, -20°C
• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen


Manuals & protocols

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