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ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit
The ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cellMás información
| Número de catálogo | Cantidad |
|---|---|
| K531000 | 1 kit |
Número de catálogo K531000
Precio (USD)
-
Cantidad:
1 kit
The ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO™ technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Typically provides 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
•Blasticidin selection
Kit Includes
• pLenti6.3⁄V5-TOPO™ TA Cloning™ Kit (K5315-20)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Typically provides 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
•Blasticidin selection
Kit Includes
• pLenti6.3⁄V5-TOPO™ TA Cloning™ Kit (K5315-20)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosHiPerform, TOPO, ViraPower
Cantidad1 kit
Tipo de productoLentiviral Expression Kit
Unit SizeEach
Contenido y almacenamiento
TOPO™ TA Cloning Reagents (Box 1)
• pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C
• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C
• 10X PCR Buffer 100 μL, -20°C
• l dNTP Mix 10 μL, -20°C
• Salt Solution 50 μL, -20°C
• CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C
• V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C
• Control PCR Template (50 ng⁄μL) 10 μL, -20°C
• Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C
• Sterile Water 1 mL
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
• Stbl3™ Cells 21 x 50 μL, -80°C
• pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C
• S.O.C. Medium 6 mL, -80°C
Support kit
• ViraPower™ Packaging Mix 195 μg, -20°C
• Lipofectamine™ 2000 0.75 mL, +4°C
• Blasticidin 50 mg, -20°C
• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen
• pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C
• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C
• 10X PCR Buffer 100 μL, -20°C
• l dNTP Mix 10 μL, -20°C
• Salt Solution 50 μL, -20°C
• CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C
• V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C
• Control PCR Template (50 ng⁄μL) 10 μL, -20°C
• Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C
• Sterile Water 1 mL
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
• Stbl3™ Cells 21 x 50 μL, -80°C
• pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C
• S.O.C. Medium 6 mL, -80°C
Support kit
• ViraPower™ Packaging Mix 195 μg, -20°C
• Lipofectamine™ 2000 0.75 mL, +4°C
• Blasticidin 50 mg, -20°C
• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen