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Invitrogen™
ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit
The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.
| Catalog Number | Quantity |
|---|---|
| K533000 also known as K5330-00 | 1 kit |
Catalog number K533000
also known as K5330-00
Price (USD)
3,455.00
Each
Quantity:
1 kit
Price (USD)
3,455.00
Each
The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's™ ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
- Stable expression
- Long-term experiments
- Accurate titer of functional virus
- Flexible and versatile Gateway™ recombination cloning technology
Key Features
- HiPerform™ WPRE and cPPT elements
- CMV promoter
- V5 epitope tag at C terminus
- Blasticidin selection
Kit includes
- pLenti6.3/V5-DEST™ Gateway™ Vector Kit (V533-06)
- ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
- 293FT Cell Line (R700-07)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodGateway
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
For Use With (Application)Protein Expression
For Use With (Equipment)HiPerform™, TOPO ™ , ViraPower™
Key FunctionsViral Expression
Product LineGateway, HiPerform, ViraPower
Product TypeExpression Kit
Protein TagV5 Epitope Tag
Quantity1 kit
Selection Agent (Eukaryotic)Blasticidin
Special ElementsWPRE, cPPT
Cell Type293-FT
FormatKit
PromoterCMV
VectorpLenti, TOPO-TA Vector
Unit SizeEach
Contents & Storage
Vector kit
• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C
• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C
• One Shot™ Stbl3™ Chemically Competent E. coli, 21 x 50 μL, store at -80°C
• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C
• S.O.C. Medium, 6 mL, store at -80°C
Support kit
• ViraPower™ Packaging Mix, 195 μg, store at -20°C
• Lipofectamine™ 2000, 0.75 mL, store at 4°C
• Blasticidin, 50 mg, store at -20°C
• 293 FT cells (3 x 106 cells/mL), 1 mL, store in liquid nitrogen