ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit

Catalog number: K533000

Invitrogen™  Related applications: Protein Expression

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The ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s™ ViraPower™ HiPerform™ Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway® recombination cloning technology

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• pLenti6.3⁄V5-DEST™ Gateway® Vector Kit (V533-06)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Viral Expression
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Product Size: 1 kit
Special Elements: WPRE, cPPT
Constitutive or Inducible System: Constitutive

Contents & storage

Vector kit
pLenti6.3⁄V5-DEST™ (150 ng⁄µL): 40 µL, store at -20°C
pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 µg⁄µL): 20 µL, store at -20°C
One Shot® Stbl3™ Chemically Competent E. coli 21 x 50 µL, store at -80°C
pUC19 control DNA (10 pg⁄µL) 50 µL, store at -80°C
S.O.C. Medium 6 mL, store at -80°C

Support kit
ViraPower™ Packaging Mix: 195 µg, store at -20°C
Lipofectamine® 2000: 0.75 mL, store at +4°C
Blasticidin: 50 mg, store at -20°C
293 FT cells (3 x 106 cells⁄mL): 1 mL, store in liquid nitrogen


Manuals & protocols

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