Flp-In™ Complete System

Catalog number: K601001

Invitrogen™  Related applications: Protein Expression

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Description

Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site.

The major components of the Flp-In™ System include:

• A Flp-In. target site vector, pFRT⁄lacZeo, for generation of a host cell line containing an integrated FRT site
• An expression plasmid containing a FRT site linked to the hygromycin resistance gene for Flp recombinase-mediated integration and selection of a stable cell line expressing your gene of interest under the control of the human cytomegalovirus (CMV) immediate-early enhancer⁄promoter
• A Flp recombinase expression plasmid, pOG44, for expression of the Flp recombinase under the control of the human CMV promoter
• A control expression plasmid containing the chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into your Flp-In. host cell line, expresses CAT
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Stable Cell Line Development, Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Hygromycin, Zeocin™
Vector Type: pFRT
Constitutive or Inducible System: Constitutive
Product Size: 1 kit

Contents & storage

The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols