Thermo Scientific™

IMAGEN™ Adenovirus Kit using Direct Immunofluorescence Assay

Catalog number: K610011-2
Thermo Scientific™

IMAGEN™ Adenovirus Kit using Direct Immunofluorescence Assay

Catalog number: K610011-2
Rapidly detect adenovirus antigen in clinical samples and confirm adenovirus in cell cultures with Thermo Scientific™ IMAGEN™ Adenovirus Kit. This direct immunofluorescence assay utilizes a genus-specific monoclonal antibody to detect an epitope of adenovirus hexon proteins, which is expressed in all known human adenovirus serotypes1.
 
Catalog Number
K610011-2
Unit Size
Each
Price (USD)
Full specifications
DescriptionIMAGEN™ Adenovirus Kit
Detectable AnalytesAdenovirus antigen
IncludesPositive control slide, mounting fluid, adenovirus reagent, instructions for use
Quantity50 Tests
Unit SizeEach
Showing 1 of 1
Catalog NumberSpecificationsUnit SizePrice (USD)
K610011-2Full specifications
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DescriptionIMAGEN™ Adenovirus Kit
Detectable AnalytesAdenovirus antigen
IncludesPositive control slide, mounting fluid, adenovirus reagent, instructions for use
Quantity50 Tests
Unit SizeEach
Showing 1 of 1

Human adenoviruses are associated with a wide range of clinical diseases in both immunocompetent and immunocompromised individuals, which include infections of the respiratory tract, conjunctiva and gastrointestinal tract2,3. Infections are common in children and can occur sporadically or in outbreaks.

Use IMAGEN Adenovirus Kit as a rapid, sensitive and specific method for direct detection and identification of human adenovirus serotypes in clinical specimens or cell cultures.

  • Rapid results—within little over 30 minutes of receiving test sample or culture
  • Simple—ready-to-use reagents plus all IMAGEN assays follow the same basic methodology
  • Excellent sensitivity and specificity—high quality, well characterized antibodies used in the assay provide excellent sensitivity and specificity
  • Easy-to-read results
  • Long shelf life—24 months

Adenoviruses are non-enveloped DNA viruses of icosahedral symmetry. The family Adenoviridae comprises two genera, mammalian adenoviruses (Mastadenoviruses) and avian adenoviruses (Aviadenoviruses)4. At least 47 known serotypes of human adenovirus have been identified and characterized by hemagglutination, neutralization, DNA-hybridization and restriction endonuclease analysis of adenoviral DNA2,4,5.

The laboratory diagnosis of adenovirus infection plays an important role in patient management and enables effective management and control of outbreaks. A range of techniques have been used to confirm the identification of adenovirus isolates, including neutralization tests, radioimmunoassay, DNA hybridization, electron microscopy and DNA electrophoretyping6,7,8,9,10. These techniques can be complex, laborious and often inappropriate for routine use. Recently indirect immunofluorescence tests or enzyme immunoassays (e.g., IDEIA™ PCE Chlamydia Kit) using genus-specific monoclonal or polyclonal antibodies have been described for the direct detection of adenovirus in clinical specimens or cell culture monolayers10,11.

Direct immunofluorescence tests utilizing specific monoclonal antibodies offer a rapid, sensitive and specific method for the direct detection of adenoviruses in clinical specimens such as nasopharyngeal aspirates and conjunctival smears or for the confirmation of adenovirus isolates in cell culture monolayers. IMAGEN Adenovirus Kit is a direct immunofluorescence test for the detection and identification of human adenovirus serotypes in clinical specimens or cell cultures.

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For In Vitro Diagnostic Use.  
  1. Cepko, C.L., Whetstone, C.A. and Sharp, P.A. (1983) Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. Journal of Clinical Microbiology 17: 360-364.
  2. Adenoviruses. In Principles and Practice of Clinical Virology (eds A.J. Zuckerman et al) John Wiley and Sons Ltd, Chapter 4 iv, pp 267-287.
  3. Horwitz, M.S. (1985) Adenoviral diseases in Virology, Raven Press, New York (eds B.N. Fields et al) pp 477-495.
  4. Frankl, R.I.B., Fauquet, C.M., Knudson, D.L., and Brown, F. (1992) Classification and Nomenclature of Viruses. Fifth Reportof the International Committee on Taxonomy of Viruses. Archives of Virology Supplement 2, Spurger Velacy, New York, pp 140-144.
  5. Albert, M.J. (1986) Enteric Adenoviruses. Archives of Virology 88, 1-17.
  6. Miller, S.E., (1986) Detection and identification of viruses by electron microscopy. Journal of Electron Microscopy Technique 4: 265-301.
  7. Darougar, S., Walpita, P., Thaker, U., Viswalingham, N., and Wishart, M.S. (1984) Rapid culture test for adenovirus isolation. British Journal of Ophthalmology 68: 405-408.
  8. Kidd, A.H., Harley, E.H., and Erasmus, M.J. (1985) Specific detection and typing of adenovirus types 40 and 41 in stool specimens by dot-blot hybridization. J. Clinical Microbiology 22: 934-939.
  9. Gomes, S.A., Nascimento, J.P., Siquera, M.M., Krawczuk, M.M., Pereira, H-G., and Russel W.C. (1985) In situ hybridization and biotinylated DNA probes: a rapid diagnostic kit for adenovirus upper respiratory infections. Journal of Virological Methods 12: 105-110.
  10. Lehtomaki, K., Julkunen I., Sandelin, K., Salonen, J., Virtanen, M., Ranki, M, and Hovi, T. (1986) Rapid diagnosis of respiratory adenovirus infections in young adult men. J Clin Microbiol. 1986 Jul;24(1):108-11.
  11. Pereira, H.G., Azeredo, R.S., Leite, J.P.G., Andrade, Z.P., and De Castro, L. (1985) A combined enzyme immunoassay for Rotavirus and Adenovirus. Journal of Virological Methods 10: 21-28.
  12. August, M. J., and Warford, A.L. (1987) Evaluation of a commercial monocloncal antibody for detection of adenovirus antigen. Journal of Clinical Microbiology 25, No 11: 2233-2235.

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