Invitrogen™

Champion™ pET302/NT-His and pET303/CT-His Vector Kit

Catalog number:  K630203

Related applications:

Protein Expression

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Description

The Champion™ pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia™ E. coli Expression Medium.


Contents and Storage:

The Champion™ pET302/NT-His and pET303/CT-His Vector Kit includes 6 µg each of pET302/NT-His and pET303/CT-His and 10 µg of a control vector. Store at -20“C. All components are guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Product Line: Champion™
Product Size: 10 µg
Promoter: T7
Protein Tag or Fusion: His Tag (6x)
Selection Agent (Eukaryotic): None
Vector Type: pDEST,pET