Thermo Scientific™

RAPID™ Hp StAR™ Lateral Flow Kit using Immunochromatographic Assay

 Related applications:
Clinical Microbiology

Rapidly detect Helicobacter pylori antigen direct from fecal samples using the Thermo Scientific™ RAPID Hp StAR™ Lateral Flow Kit using Immunochromatographic Assay. The test utilizes the same highly specific monoclonal antibodies used in Thermo Scientific™ Amplified IDEIA Hp StAR™ (K663011-2) combined with an amplified capture reagent to give enhanced sensitivity and specificity.

RAPID Hp StAR Lateral Flow Kit can be used to aid the diagnosis of H.pylori infection in adults and children and can also be used to monitor eradication therapy.

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Description

Helicobacter pylori infection is one of the most common causes of gastritis, and is involved in gastric ulcer, duodenal ulcer and gastric adenocarcinoma. It is also the causative agent in a high percentage of dyspepsia cases.

H.pylori bacteria have adapted to live in the acidic environment of the stomach. The enzyme urease cleaves urea into ammonia and carbon dioxide, thus neutralizing the acid and enabling H. pylori to survive the bactericidal conditions of the stomach. The production of catalase and superoxide dismutase protects the bacteria from neutrophilic attack in the stomach mucosa1.

Use RAPIDTM Hp StARTM Lateral flow Kit for the initial diagnosis of H.pylori infections as well as for monitoring eradication success four to six weeks after completion of eradication therapy and also for the diagnosis of reinfection. 

    • Rapid results - within 15 minutes of receiving test sample
    • Simple- ready-to-use reagents plus all the assays follow the same basic methodology
    • Excellent sensitivity and specificity- high quality, well-characterized antibodies used in the assay provide excellent sensitivity and specificity
    • Easy-to- read results
    • Long Shelf Life- typically 24 months.

An amplified capture reagent incorporating streptavidin is bound to a nitrocellulose membrane. Two different monoclonal antibodies specific to H. pylori antigen are dried onto another membrane at the base of the strip. One of these monoclonal antibodies is conjugated to colloidal gold, and the other is conjugated to biotin. When the strip is dipped into the diluted stool sample, the liquid sample moves up the strip by capillary action, solubilising the two monoclonal antibody conjugates. Any H. pylori antigen present in the sample will bind to the two solubilised monoclonal antibody conjugates to form an antigen-antibody complex. Each complex will consist of antigen, bound to both monoclonal antibody conjugated to colloidal gold, and monoclonal antibody conjugated to biotin. This complex migrates further up the strip until it reaches the streptavidin capture reagent, which captures the biotin element of the antigen-antibody complex, immobilizing the whole complex and forming a purple-pink line. Remaining unbound colloidal gold conjugate continues migrating up the strip and is captured by an anti-mouse polyclonal antibody that is immobilised on the control area of the nitrocellulose, forming a second purple-pink line, indicating that the test has performed correctly.

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1.D’Elios, M.M., Andersen, L.P., Del Prete, G. (1998). Inflammation and host response. Current Opinion in Gastroenterology 14 (suppl. 1): 15-19.



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