InsectSelect™ Glow Kit with Sf9 Cells

Catalog number: K81001

Invitrogen™  Related applications: Protein Expression

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Description

The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your
gene of interest. This vector has the following features:

• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin

In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: OplE2
Vector Type: pIZ
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Protein Tag Position (to your gene): C-terminal

Contents & storage

The InsectSelect™ Glow Kit includes 20 µg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 µg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin® Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin® Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols