Invitrogen™
InsectSelect™ Glow Kit with Sf9 Cells
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Description
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| Cloning Method: | Restriction Enzyme ⁄ MCS |
|---|---|
| Product Line: | InsectSelect™ |
| Product Size: | 1 kit |
| Promoter: | OplE2 |
| Protein Tag Position (to your gene): | C-terminal |
| Protein Tag or Fusion: | His Tag (6x), V5 Epitope Tag |
| Vector Type: | pIZ |
Contents & storage
The InsectSelect™ Glow Kit includes 20 µg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 µg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin® Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin® Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
