The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression • The Zeocin™ resistance gene for rapid selection of stably transfected cell lines • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
For Research Use Only. Not for use in diagnostic procedures.
The InsectSelect™ Glow Kit includes 20 µg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 µg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin® Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin® Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.