Invitrogen™

Bac-N-Blue™ Transfection Kit

Catalog number:  K85501

Related applications:

Protein Expression

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Description

The Bac-N-Blue™ Linear DNA was specifically designed for recombination with the pBlueBac vectors and pMelBac. Recombinant viruses have a full-length, functional lacZ gene resulting in the production of
blue plaques. This allows for easy identification and purification. Bac-N-Blue™ DNA can be used with any polyhedrin promoter-based baculovirus transfer vector. Bac-N-Blue™ DNA is linearized at three
sites, one of which is in a gene that is essential for viral propagation (1). This leads to a decrease in non-recombinant virus, making selection and purification of recombinant virus easy. In addition to the viral DNA, the Bac-N-Blue™ Transfection Kit contains Cellfectin® Reagent for high transfection efficiency.
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Cloning Method: Restriction Enzyme ⁄ MCS
Product Line: Bac-N-Blue™
Product Size: 5 transfections
Promoter: Polyhedrin
Protein Tag or Fusion: Untagged
Vector Type: pBlueBac

Contents & storage

The Bac-N-Blue™ Transfection Kit contains five 0.5 µg vials of Bac-NBlue™ linear virus DNA and 125 µl of Cellfectin® Reagent.
Store at +4°C. All components are guaranteed stable for 6 months when properly stored.