FreeStyle™ 293 Expression System

      Catalog number:  K900001

      Invitrogen™  Related applications: Antibody & Cell Culture Production | Protein Expression | Transfection

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      Description

      The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.

      FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.
      For Research Use Only. Not for use in diagnostic procedures.

      Figures

      Specifications

      Cell Line: FreeStyle™ 293-F Cells
      Classification: Serum-Free
      Culture Type: Suspension Cell Culture
      Delivery Method: Transfection
      Expression: Mammalian
      Expression Mechanism: Cell-Based Expression
      Key Function: Protein Production
      Product Line: Freestyle™
      Product Size: 1 kit

      Contents & storage

      The FreeStyle™ 293 Expression System contains:
      • 1L GIBCO® FreeStyle™ 293 Expression Medium
      • 1 x 107 FreeStyle™ 293-F Cells (frozen)
      • 1 mL 293fectin™ Transfection Reagent
      • 100 mL Opti-MEM® I for Complex Formation
      • 25 µg pCMV-SPORT β-gal
      Please review the FreeStyle™ 293 System Manual for storage and handling information. Store FreeStyle™ 293-F Cells in liquid nitrogen. Store 293fectin™ Transfection Reagents at 4°C. Store GIBCO® FreeStyle™ 293 Expression Medium and Opti-MEM® I at 2-8°C in the dark. Store pCMV SPORT β-gal at -20°C. Reagents are guaranteed stable for six months when stored properly.

      Documents

      Manuals & protocols