Invitrogen™
FreeStyle™ 293 Expression System
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Description
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Line: | FreeStyle™ 293-F Cells |
|---|---|
| Classification: | Serum-Free |
| Culture Type: | Suspension Cell Culture |
| Delivery Method: | Transfection |
| Expression: | Mammalian |
| Expression Mechanism: | Cell-Based Expression |
| Key Function: | Protein Production |
| Product Line: | Freestyle™ |
| Product Size: | 1 kit |
Contents & storage
The FreeStyle™ 293 Expression System contains:
- 1L GIBCO® FreeStyle™ 293 Expression Medium
- 1 x 107 FreeStyle™ 293-F Cells (frozen)
- 1 mL 293fectin™ Transfection Reagent
- 100 mL Opti-MEM® I for Complex Formation
- 25 µg pCMV-SPORT β-gal
