FreeStyle™ MAX 293 Expression System
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Gibco™

FreeStyle™ MAX 293 Expression System

The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle深入閱讀
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產品號碼Quantity
K9000101 Kit
產品號碼 K900010
價格 (TWD)
-
Quantity:
1 Kit
The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production

The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.

For Research Use Only. Not for use in diagnostic procedures.
規格
Cell LineFreeStyle™ 293-F Cells
ClassificationAnimal Origin-Free, Serum-Free
Culture TypeSuspension Cell Culture
Delivery TypeTransfection
Expression MechanismCell-Based Expression
Expression SystemMammalian
For Use With (Application)Protein Expression
Key FunctionsProtein Production
Product LineFreestyle
Product TypeExpression System
Quantity1 Kit
Cell Type293-derived Cells
Transfection TechniqueLipid-based Transfection, Transient Transfection
Unit SizeEach
內容物與存放
Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.

常見問答集 (常見問題)

What is the main difference between the FreeStyle Max 293 Expression System and the FreeStyle 293 Expression System?

The two systems have the same components except for the transfection reagent and the medium supplied for formation of transfection complexes. The FreeStyle Max 293 Expression System contains FreeStyle Max Reagent and OptiPRO SFM (serum-free medium that is devoid of animal-origin components), whereas the FreeStyle 293 Expression System contains 293fectin Transfection Reagent and Opti-MEM I Reduced Serum Medium (serum-free medium but not animal-origin free).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the recommended ratio of DNA and transfection reagent when transfecting Freestyle cells?

The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Which vectors do you recommend for use with FreeStyle system?

Generally, any mammalian expression vector should be compatible with the FreeStyle expression system. Some of the more popular choices include pcDNA3.3-TOPO TA vector (Cat. No. K8300-01), pcDNA3.4-TOPO TA vector (Cat. no. A14697), pcDNA3.2 DEST vector (Cat. No. 12489018), pCEP vector (Cat. No. V044-50), or pcDNA4/HisMax vector (Cat. No. K864-20).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does FreeStyle MAX reagent compare to Lipofectamine 2000 and 293fectin reagents?

Lipofectamine 2000 is designed for adherent cells. FreeStyle and 293fectin reagents are designed for suspended Hek293 and CHO cells. For transfecting 293 suspension cells, FreeStyle MAX reagent yields transfection efficiency comparable to Lipofectamine 2000 and 293fectin reagents. However, Lipofectamine 2000 and 293fectin reagents do not work well for CHO suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What's the transfection efficiency for FreeStyle MAX reagent?

In general, the transfection efficiency for FreeStyle MAX reagent is 65% when used with suspension CHO and 293 cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.