Ni-NTA Purification System
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Zitierungen und Referenzen (4)
Invitrogen™

Ni-NTA Purification System

Das Ni-NTA Aufreinigungssystem wurde für die Aufreinigung rekombinanter Proteine entwickelt, die eine Polyhistidin (6xHis)-Sequenz enthalten. Das Kit verwendet Ni-NTA Nickel-ChelatharzWeitere Informationen
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KatalognummerMenge
K950016 Aufreinigungen
Katalognummer K95001
Preis (EUR)
1.390,00
Each
Zum Warenkorb hinzufügen
Menge:
6 Aufreinigungen
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
1.390,00
Each
Zum Warenkorb hinzufügen
Das Ni-NTA Aufreinigungssystem wurde für die Aufreinigung rekombinanter Proteine entwickelt, die eine Polyhistidin (6xHis)-Sequenz enthalten. Das Kit verwendet Ni-NTA Nickel-Chelatharz und wird mit nativen und denaturierenden Puffern zur effizienten Reinigung rekombinanter Proteine unter verschiedenen Bedingungen geliefert.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
FormatSuspension
ProdukttypNi-NTA-Aufreinigungssystem
Menge6 Aufreinigungen
ProteinmarkierungHis-Tag
Unit SizeEach
Inhalt und Lagerung
Sechs 2 ml-Harzsäulen und Puffer für die native und denaturierende Aufreinigung. Zehn Milliliter Ni-NTA Agarose. Bei +4 °C lagern. Alle Reagenzien bleiben bei ordnungsgemäßer Lagerung garantiert 6 Monate stabil.

Häufig gestellte Fragen (FAQ)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (4)

Zitierungen und Referenzen
Abstract
A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.
Authors:Howard PW, Maurer RA
Journal:J Biol Chem
PubMed ID:7673116
'Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription. Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined. Recent reports suggest that phosphorylation of ... More
Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).
Authors: Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;
Journal:J Biol Chem
PubMed ID:10956661
'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More
Probable Identification of a Membrane-Associated Repressor of Bacillus subtilis DNA Replication as the E2 Subunit of the Pyruvate Dehydrogenase Complex
Authors:Andrew Stein and William Firshein*
Journal:J Bacteriol
PubMed ID:10735853
Two Bacillus subtilis lysogenic libraries were probed by an antibody specific for apreviously described membrane-associated inhibitor of B. subtilis DNA replication(J. Laffan and W. Firshein, Proc. Natl. Acad. Sci. USA 85:7452-7456, 1988). Threeclones that reacted strongly with the antibody contained an entire open reading frame.Sequencing identified one of the clones ... More
Sox9 and p300 cooperatively regulate chromatin-mediated transcription.
Authors:Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,
Journal:J Biol Chem
PubMed ID:16109717
Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More