Invitrogen™

Ni-NTA Purification System with Antibody, with Anti-His (C-term)-HRP Antibody

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence.

Catalog Number	Quantity
K95301 also known as K953-01	1 Kit

Catalog number K95301

also known as K953-01

Price (USD)

Quantity:

1 Kit

Request bulk or custom format

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity1 Kit

TypeProtein Purification System

Unit SizeEach

Contents & Storage

Six 2-ml resin columns and buffers for native and denaturing purification. Ten milliliters of Ni-NTA Agarose. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.