pEF6/V5-His TOPO® TA Expression Kit

Catalog number: K961020

Invitrogen™  Related applications: Protein Expression

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Description

The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO® Cloning. The pEF6/V5-His-TOPO® vector includes:

Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells

• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: EF-1α
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: TOPO®-TA
Selection Agent (Eukaryotic): Blasticidin
Vector Type: TOPO-TA Vectors, pEF
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Constitutive
Product Size: 20 reactions

Contents & storage

Each pEF6/V5-His TOPO® TA Expression Kit contains two boxes. The TOPO® TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO® vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot® Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-µl aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.

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