Invitrogen™

pEF6/V5-His TOPO™ TA Expression Kit

Catalog number: K961020
Invitrogen™

pEF6/V5-His TOPO™ TA Expression Kit

Catalog number: K961020
Catalog Number
K961020
also known as K9610-20
Unit Size
20 reactions
Price (USD)
1,018.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K961020
also known as K9610-20
20 reactions
1,018.00
Your Price:
***
Product Overview
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The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO® Cloning. The pEF6/V5-His-TOPO® vector includes:

Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells

• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Constitutive Expression
Product Line
TOPO™
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
EF-1α
Product Type
TOPO TA Expression Kit
Selection Agent (Eukaryotic)
Blasticidin
Cleavage
EK (Enterokinase) Recognition Site
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
TOPO™-TA
Vector
pEF, TOPO-TA Vectors

Contents & Storage

Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-µl aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.

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