This product is equivalent to the Thermo Scientific™ Pierce™ Human IFN alpha ELISA Kit (Supernatant)

IFN alpha ELISA Kit, Human

Catalog number: KHC4011

 Related applications: Protein Assays and Analysis

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The IFNα Human ELISA kit is designed to detect and quantify the level of human interferon alpha (IFNα) in media using 96-well plates and a microplate reader. The IFNα ELISA kit is manufactured by PBL Assay Science. This is an alternate product for Cat. No. KHC4012.

Easy-to-run sandwich ELISA

• Results typically in one day—rapid 3 hr, 15 min incubation protocol
• Pre-coated removable 8-well strips
• Rigorous validation

Get Accuracy with Simplicity
The IFNα Human ELISA Kit utilizes a straightforward, rapid protocol with a total assay incubation time of only 3 hours and 15 minutes. A capture antibody specific for IFNα has been coated onto the wells of the 96-well plate provided. At the beginning of the first incubation, standards of known IFNα content and unknown samples are pipetted into the wells where the IFNα antigen binds to the immobilized capture antibody. After washing, detection antibody is added to the wells. During the second incubation, the detection antibody binds to the immobilized IFNα protein captured during the first incubation. After washing, HRP solution is added; this binds to the detection antibody to complete the four member sandwich. Following a third incubation and wash step to remove all unbound enzyme, a substrate solution (TMB) is added. The bound enzyme acts upon it to produce a colored reaction product. The optical density of the color is directly proportional to the concentration of IFNα present in the original specimen and can be read on a standard microplate reader.

The Ready-to-Use IFNα Human ELISA Kit Contains These Reagents, Buffers, and Supplies:
• Antibody Coated 96-Well Plate
• Human IFNα Standard
• Diluent Buffer
• Detection Antibody Concentrate
• Horseradish Peroxidase (HRP) Conjugate Concentrate
• HRP Diluent
• Wash Buffer Concentrate
• Stabilized TMB Chromogen
• Stop Solution
• Plate Covers

Get Results Quickly and Economically
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to get quantitative results off the standard curve and/or as a positive control.

Part of the Life Technologies ELISA Kit Family—Your Assurance of Quality
We have a broad menu of ELISA kits targeted to disease-related proteins. With excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

Learn more about ELISA Kits
Learn more about other Immunoassays

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Gene Aliases: IFN-alphaA, IFNA, IFNA2B, INFA2, MGC125764, MGC125765
Accession Number: NM_000605.3, NP_000596.2
Shipping Condition: Wet Ice
Marker: IFN-α
Gene ID: 3440
Species: Human
Gene Symbol: IFNA2
Sensitivity: 13 pg⁄ml
Target Gene: interferon, alpha 2
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 assays
Sample Volume: 100 µl
Protein Family: Cytokines & Receptors
Incubation Time: 195 min
Detection Method: Colorimetric
Research Category: Immunology
Sample Type (Specific): Plasma, Serum, Supernatants
For Use With (Equipment): Microplate Reader

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.


Manuals & protocols