Caspase Colorimetric Sampler Kit

Catalog number: KHZ1001

Invitrogen™  Related applications: Cell Viability, Proliferation & Function | Protein Assays and Analysis

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The ApoTarget™ Caspase Colorimetric Protease Assay Sampler Kit provides all reagents required for the detection of caspase proteolytic activity in lysates of mammalian cells. The kit provides a simple and convenient means for quantitating the enzyme activity of caspases that recognize the amino acid sequences VDVAD (caspase-2), DEVD (caspase-3), VEID (caspase-6), IETD (caspase-8), and LEHD (caspase-9). The individual substrates provided for measuring the activity of these caspases are synthetic peptides that are labeled at their C-termini with para-nitroaniline (pNA). Upon cleavage of the substrates by caspases, absorption of light by free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase activity.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader
Format: 96-well plate
Label or Dye: pNA
Product Line: ApoTarget™
Product Size: 125 tests
Shipping Condition: Dry Ice

Contents & storage

Contains Lysis buffer, substrate, reaction buffer, reducing agent, and complete protocol


Manuals & protocols

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