NGAL Rapid ELISA Kit, Human

Catalog number: KIT037

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Rapidly assay NGAL (human neutrophil gelatinase-associated lipocalin) levels in human serum, plasma, and urine samples with our Thermo Scientific Human NGAL Rapid ELISA Kit.

Features of the Human NGAL Rapid ELISA Kit:

Target: natural and recombinant human NGAL (human neutrophil gelatinase-associated lipocalin)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450 nm minus absorbance at 550nm
Assay Range: 0.2-20 ng/mL (= at least 5 ng/mL NGAL in original, undiluted samples)
Sensitivity: < 100 pg/mL
Precision: undetermined
Sample Types: urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 1 hour
Sample Size: 50 µL of 25-fold diluted sample per well
Specificity: The two monoclonal antibodies against human NGAL used in the assay have been shown to bind to different preparations of recombinant human NGAL and to give a single band at 25kDa on Western blot analysis of a reduced postnuclear supernatant from human neutrophils.

This human NGAL ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of neutrophil gelatinase-associated lipocalin (NGAL). The assay is an ELISA performed in microplate wells coated with a monoclonal antibody against human NGAL. Bound NGAL is detected with a horseradish peroxidase (HRP)-conjugated monoclonal antibody and the assay is developed by incubation with a color-forming substrate. The assay is a rapid two-step procedure.

NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are typically small secreted proteins characterized by their ability to bind small, hydrophobic molecules in a structurally conserved pocket formed by beta-pleated sheet, to bind to specific cell-surface receptors and to form macromolecular complexes. NGAL has many synonyms: it also known as NL (neutrophil lipocalin; HNL: human NL), lipocalin 2, oncogene protein 24p3 or uterocalin (in the mouse) and neu-related lipocalin or 25kDa alpha-2-microglobulin-related protein (in the rat). Human NGAL consists of a single disulfidebridged polypeptide chain of 178 amino-acid residues with a calculated molecular mass of 22kDa, but glycosylation increases its apparent molecular mass to 25kDa. In neutrophils (neutrophilic polymorphonuclear leukocytes) and urine it occurs as monomer, with a small percentage of dimer and trimer, and also in complex with 92-kDa human neutrophil type IV collagenase, also called gelatinase B or matrix metalloproteinase-9 (MMP-9). NGAL is often used as a marker of acute renal injury which may lead to acute renal failure.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Human
Product Size: 96 assays
Protein Family: Growth Factors & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody(s), diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols