Applied Biosystems™

Histogene™ LCM Frozen Section Staining Kit, includes dehydration chemicals, stain, and slides

Catalog number:  KIT0401

 Related applications:

Laser Capture Microdissection

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Description

The Arcturus® HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for laser capture microdissection (LCM). Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

Key product features:
• Optimized process—provide superior staining while preserving RNA
• Comprehensive kit—simplify tissue staining and dehydration
• High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity
• Versatile application—obtain high-quality RNA from many tissue types
• Modular design—use with other Arcturus products to produce superior microarray data

Provide Superior Staining while Preserving Nucleic Acids
HistoGene® Staining Solution has been developed by Arcturus® to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene® stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.

Simplify Tissue Staining and Dehydration
The HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for LCM.

Retain Low-abundance mRNA
RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene® LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene® samples appears free of degradation.

Maintain RNA Integrity
Tissues prepared for LCM using HistoGene® LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.

Obtain High-quality RNA from Many Tissue Types
Arcturus® has validated the HistoGene® Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene® Kit have yielded quality RNA. Validated tissue types include (Table 1):

Part of the Arcturus® System for Microgenomics
The HistoGene® LCM Frozen Section Staining Kit is part of the Arcturus® Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure® RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. Arcturus® instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Downstream Application: Laser Capture Microdissection (LCM)
For Use With (Equipment): ArcturusXT™ LCM Instrument
Product Line: Histogene®
Product Size: 72 applications
Sample Type (General): Cells, Tissue
Sample Type (Specific): Tissue (Frozen)
Workflow Step: Tissue Staining

Contents & storage

HistoGene® Staining Solution, ethanols (100%, 95%, 75%), xylenes, slide staining jars, HistoGene® LCM slides. Store at room temperature

Documents

Manuals & protocols