TA Cloning® Kit, with pCR™2.1 Vector and One Shot® TOP10F' Chemically Competent E. coli, no manual

TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR™ vector and yield ≥80% recombinants with ≥90% of the recombinants containing inserts.

Some of the convenient features of the pCR™ vectors are:

• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1) or T7 and Sp6 (pCR™II) promoters for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of inserts
• Choice of kanamycin or ampicillin selection
• Easy blue/white colony screening
• M13 forward and reverse primer sites for sequencing