Sal Fast Blue BB
Sal Fast Blue BB
Sal Fast Blue BB
Thermo Scientific Chemicals

Sal Fast Blue BB

CAS: 5486-84-0 | C34H36Cl4N6O6Zn | 831.878 g/mol
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Número de catálogoCantidad
L09704.03
también denominado L09704-03
1 g
Número de catálogo L09704.03
también denominado L09704-03
Precio (USD)
-
Cantidad:
1 g
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Identificadores químicos
CAS5486-84-0
IUPAC Name4-benzamido-2,5-diethoxybenzene-1-diazonium
Molecular FormulaC17H18N3O3
InChI KeyDKPBKNZQVUOHRQ-UHFFFAOYSA-O
SMILESCCOC1=CC([N+]#N)=C(OCC)C=C1NC(=O)C1=CC=CC=C1
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EspecificacionesSpecification SheetHoja de especificaciones
Appearance (Color)Yellow to green or dark brown
Formpowder
Assay (unspecified)> 80.0% dye content
Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Fast Blue BB salt is used as a general substrate for β-glycosidase. Two moles of 6-bromo-2-napthol-β-d-glucopyranoside are cleaved by β-glucosidases into 2 moles of d-glucose and 2 moles of 6-bromo-2-napthol. The 2 moles of 6-bromo-2-napthol formed are coupled with 1 mole of Fast Blue BB salt [4-benzoylamino-2,5-diethoxybenzenediazonium chloride hemi (zinc chloride) salt], resulting in 2 moles of the corresponding dye of 6-bromo-2-naphthol (reddish color). It is a commonly used stain to observe myelin under light microscopy.

Solubility
Soluble in water.
RUO – Research Use Only
  1. Helene Breitschopf.; Gerda Suchanek.; Robert M. Gould.; David R. Colman.; Hans Lassmann. In situ hybridization with digoxigenin-labeled probes: sensitive and reliable detection method applied to myelinating rat brain.Acta Neuropathologica. 1992, 84 (6),581-587 .