SuperScript® Direct cDNA Labeling System

Catalog number: L101502

Invitrogen™  Related applications: Microarray Analysis | Nucleic Acid Labeling & Oligo Synthesis

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Description

The SuperScript® Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample. In this method (Figure 1), anchored oligo(dT) anneals to the mRNA template. SuperScript® III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.

The SuperScript® Direct cDNA Labeling System uses 800 units of SuperScript® III RT to generate:

• High cDNA yields without an additional enzyme "spike" (Figure 2)
• Strong signal intensities (Figure 3)
• A fast and simple protocol that minimizes "hands on" time and provides superior reproducibility
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 647, Cy™3, Cy™5, Alexa Fluor™ 555
Product Size: 30 reactions
Final Product: cDNA (Labeled)
Labeling Method: Direct Labeling
Labeling Target: cDNA
Detection Method: Fluorescent
Includes Label or Dye: No
Sample Type (General): RNA

Contents & storage

The SuperScript® Direct cDNA Labeling Module includes SuperScript® III Reverse Transcriptase, Anchored Oligo(dT), Random Primers, control RNA, 5X First-Strand Buffer, 0.1 M DTT, 10 mM dNTP Mix, and DEPC-treated water. The SuperScript® Direct cDNA Labeling System includes all components listed for the SuperScript® cDNA Labeling Module plus purification columns, loading buffer, wash buffer, and amber collection tubes. Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols