AuthorsLong MS, Jones CD, Helfrich MR, Mangeney-Slavin LK, Keating CD
JournalProc Natl Acad Sci U S A
PubMed ID15788532
'An experimental model for cytoplasmic organization is presented. We demonstrate dynamic control over protein distribution within synthetic cells comprising a lipid bilayer membrane surrounding an aqueous polymer solution. This polymer solution generally exists as two immiscible aqueous phases. Protein partitioning between these phases leads to microcompartmentation, or heterogeneous protein distribution ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Hypocretin and nicotine excite the same thalamocortical synapses in prefrontal cortex: correlation with improved attention in rat.
AuthorsLambe EK, Olausson P, Horst NK, Taylor JR, Aghajanian GK
JournalJ Neurosci
PubMed ID15917462
Thalamic projections to prefrontal cortex are important for executive aspects of attention. Using two-photon imaging in prefrontal brain slices, we show that nicotine and the wakefulness neuropeptide hypocretin (orexin) excite the same identified synapses of the thalamocortical arousal pathway within the prefrontal cortex. Although it is known that attention can ... More
Dendritic spine heterogeneity determines afferent-specific Hebbian plasticity in the amygdala.
AuthorsHumeau Y, Herry C, Kemp N, Shaban H, Fourcaudot E, Bissière S, Lüthi A
JournalNeuron
PubMed ID15629707
Functional compartmentalization of dendrites is thought to underlie afferent-specific integration of neural activity in laminar brain structures. Here we show that in the lateral nucleus of the amygdala (LA), an area lacking apparent laminar organization, thalamic and cortical afferents converge on the same dendrites, contacting neighboring but morphologically and functionally ... More
Axonal bias at a representational border in adult rat somatosensory cortex (S1).
AuthorsSteen PA, Mason M, Pham L, Lefebvre Y, Hickmott PW
JournalJ Comp Neurol
PubMed ID17154268
The cortex is a highly organized structure and this organization is integral to cortical function. However, the circuitry underlying cortical organization is only partially understood, thus limiting our understanding of cortical function. Within the somatosensory cortex, organization is manifest as a map of the body surface. At the level of ... More
SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells.
Authors
JournalSci Adv
PubMed ID33608275
3D projection electrophoresis for single-cell immunoblotting.