Invitrogen™

LysoTracker™ Yellow HCK-123, special packaging

Catalog number: L12491
Invitrogen™

LysoTracker™ Yellow HCK-123, special packaging

Catalog number: L12491
Catalog Number
L12491
Unit Size
20 x 50 µL
Price (USD)
Price: 428.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L1249120 x 50 µL
Price: 428.00
Your Price:
***
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LysoTracker Yellow HCK-123 is a cell-permeable, non-fixable, yellow fluorescent dye that stains acidic compartments within a cell, such as lysosomes.Yellow HCK-123 has an excitation and emission maximum of 465/535 nm. LysoTracker probes are available in a variety of fluorescent colors.

Simple, highly specific, one-step staining and tracking of acidic organelles
LysoTracker probes consist of a hydrophobic fluorophore linked to a weak base that is only partially protonated at neutral pH. In a neutrally charge state, LysoTracker probes can freely diffuse across intact plasma membranes of live cells. Due to the inherently acidic properties within the lysosome, upon diffusion into the lysosome the weakly basic moiety is protonated. In this charged state, the probe does not readily diffuse across the organelle membrane, providing a localized accumulation for distinct staining of acidic organelles such as lysosomes. Effective at nanomolar concentrations, LysoTracker probes are highly selective for acidic organelles and provide simple one-step staining that does not rely on antibody detection.

Lysosomes are acidic in nature due to the production and storage of digestive enzymes (hydrolases). The acidic environment of lysosomes enables degradation of carbohydrates, lipids, nucleic acids, and peptides. Extracellular proteins, virus or bacteria can also be internalized and trafficked to the lysosome for degradation via the lysosomal proteolysis pathway. In addition, misfolded proteins or damaged organelles become targets for lysosomal degradation via the induction of autophagy. Autophagy is important for cellular differentiation, survival during nutrient deprivation, and normal growth control. The inherent acidic nature within lysosomes, and any change of pH during biosynthesis or pathogenesis, can be exploited by various probes that respond to an acidic environment. LysoTracker products provide fluorescence detection for live-cell staining of acidic environments and can be used for labeling and tracing acidic organelles such as lysosomes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Yellow
Concentration
1 mM
Excitation Wavelength Range
465/535
For Use With (Equipment)
Fluorescence Microscope, Fluorescent Imager
Detection Method
Fluorescence
Description
LysoTracker™ Yellow HCK-123
Emission
Yellow, Yellow/Orange
Form
Liquid
Product Line
LysoTracker™
Shipping Condition
Room Temperature
Label Type
Non-fixable, live cell organelle stain
Product Type
Dye
SubCellular Localization
Lysosomes

Contents & Storage

Store desiccated at ≤–20°C
• Protect from light
• Avoid freeze-thaw cycles and do not store in a frost-free freezer
• Store in single-use aliquots, if possible