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Fibroblast (3T3, COS-7) | Myoblast (C2C12, L6 CRL-1458) |
Hepatocyte (HepG2, HuH7) | Erythroleukemic cell (K562) |
Breast carcinoma (MCF7, Hs578T) | Prostate carcinoma (LNCap) |
Lung carcinoma (A549, NCI-H460) | Osteosarcoma (U-2 OS, Saos-2) |
Colon carcinoma (Caco2, SW480) | Pancreatic carcinoma (PANC-1) |
Skin melanoma (SK-MEL-28) |
Lipofectamine® 3000 transfection procedure details
Enhanced transfection efficiency using Lipofectamine® 3000
Lipofectamine® 3000 transfection workflow
Cleavage efficiency for GeneArt® TALENs and CRISPRs
Lipofectamine® 3000 Transfection Reagent delivers better transfection performance than Lipofectamine® 2000 reagent as demonstrated by a customer (Rui Eduardo Castro, PhD, University of Lisbon). Shown are the results of transfection of undifferentiated and 3-day differentiated C2C12 cells in the presence of serum and antibiotic (100 ng GFP reporter vector was used per well in 24-well plates; analyzed 48 hrs later).
Cell Type: | Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells |
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Sample Type (Specific): | Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA |
Classification: | Animal Origin-Free |
Transfection Technique: | Lipid-Based Transfection |
Serum Compatible: | Yes |
Product Line: | Lipofectamine® |
Product Size: | 0.75 mL |