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Invitrogen™

Lipofectamine™ 3000 Transfection Reagent

Catalog number: L3000015
Invitrogen™

Lipofectamine™ 3000 Transfection Reagent

Catalog number: L3000015
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
 
Catalog Number
L3000015
Unit Size
1.5 mL
Quantity
1.5 mL
Price (USD)
Price: 842.00
Online price: 783.65
Your price:
Availability
-
Full specifications
Quantity
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity1.5 mL
Serum CompatibleYes
Unit Size1.5 mL
Catalog Number
Unit Size
0.1 mL
Quantity
0.1 mL
Price (USD)
Price: 84.25
Online price: 78.65
Your price:
Availability
-
Full specifications
Quantity
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.1 mL
Serum CompatibleYes
Unit Size0.1 mL
Catalog Number
Unit Size
0.75 mL
Quantity
0.75 mL
Price (USD)
Price: 536.00
Online price: 493.65
Your price:
Availability
-
Full specifications
Quantity
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Unit Size0.75 mL
Catalog Number
Unit Size
5 x 1.5 mL
Quantity
5 x 1.5 mL
Price (USD)
Price: 4,085.00
Online price: 3,672.65
Your price:
Availability
-
Full specifications
Quantity
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity5 x 1.5 mL
Serum CompatibleYes
Unit Size5 x 1.5 mL
Catalog Number
Unit Size
15 ml
Quantity
15 mL
Price (USD)
Price: 7,300.00
Online price: 6,788.65
Your price:
Availability
-
Full specifications
Quantity
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity15 mL
Serum CompatibleYes
Unit Size15 ml
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeQuantityPrice (USD)AvailabilityQuantity
L3000015Full specifications
1.5 mL1.5 mL
Price: 842.00
Online price: 783.65
Your price:
-
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity1.5 mL
Serum CompatibleYes
Unit Size1.5 mL
L3000001Full specifications
0.1 mL0.1 mL
Price: 84.25
Online price: 78.65
Your price:
-
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.1 mL
Serum CompatibleYes
Unit Size0.1 mL
L3000008Full specifications
0.75 mL0.75 mL
Price: 536.00
Online price: 493.65
Your price:
-
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Unit Size0.75 mL
L3000075Full specifications
5 x 1.5 mL5 x 1.5 mL
Price: 4,085.00
Online price: 3,672.65
Your price:
-
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity5 x 1.5 mL
Serum CompatibleYes
Unit Size5 x 1.5 mL
L3000150Full specifications
15 ml15 mL
Price: 7,300.00
Online price: 6,788.65
Your price:
-
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity15 mL
Serum CompatibleYes
Unit Size15 ml
Showing 5 of 5
This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
  • Superior performance—our highest efficiency reagent for difficult-to-transfect cells
  • Improved cell viability—gentle on your cells, with low toxicity
  • Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.

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