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Invitrogen™

Lipofectamine™ 3000 Transfection Reagent

Green features
Catalog number: L3000015
Invitrogen™

Lipofectamine™ 3000 Transfection Reagent

Green features
Catalog number: L3000015
Catalog Number
L3000015
also known as L3000-015
Unit Size
1.5 mL
Price (USD)
692.00
Your Price:
Availability
***
Quantity
Catalog Number
L3000001
also known as L3000-001
Unit Size
0.1 mL
Price (USD)
69.25
Your Price:
Availability
***
Quantity
Catalog Number
L3000008
also known as L3000-008
Unit Size
0.75 mL
Price (USD)
438.00
Your Price:
Availability
***
Quantity
Catalog Number
L3000075
also known as L3000-075
Unit Size
5 x 1.5 mL
Price (USD)
3,350.00
Your Price:
Availability
***
Quantity
Catalog Number
L3000150
also known as L3000-150
Unit Size
15 ml
Price (USD)
5,990.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L3000015
also known as L3000-015
1.5 mL
692.00
Your Price:
***
L3000001
also known as L3000-001
0.1 mL
69.25
Your Price:
***
L3000008
also known as L3000-008
0.75 mL
438.00
Your Price:
***
L3000075
also known as L3000-075
5 x 1.5 mL
3,350.00
Your Price:
***
L3000150
also known as L3000-150
15 ml
5,990.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28) 


View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Specifications

Format
24-Well Plate
Product Type
Transfection Reagent
Quantity
1.5mL
Classification
Animal Origin-Free
Serum Compatible
Yes
Product Line
Lipofectamine™
For Use With (Application)
Transfection
Sample Type
Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Green Features
Sustainable packaging
Transfection Technique
Lipid-Based Transfection
Cell Type
Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells

Contents & Storage

Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.

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