Lipofectamine® 3000 Transfection Reagent

Catalog number: L3000150

Invitrogen™  Related applications: Transfection

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Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28) 

View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine® 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine® 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine® 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Established Cell Lines, Primary Cells, Stem Cells, Hard-to-Transfect Cells
Sample Type (Specific): Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Classification: Animal Origin-Free
Transfection Technique: Lipid-Based Transfection
Serum Compatible: Yes
Product Size: 15 ml

Contents & storage

Contains one vial of Lipofectamine® 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.


Manuals & protocols

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