LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Green/Deep Red) - FAQs

We have not validated the use of LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)(Cat. No. L32250) on bacteria, fungi or protozoa. In general, calcein, AM does not work well with bacteria due to their low intracellular esterase activity. Some protozoa may readily engulf cell impermeant stains and result in intracellular staining of live cells.

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If an expiration date or warranty period is not posted on any public-facing document, the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. Nos. L32250) have the standard warranty period of 1 year from the date of shipment.

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The formulation/identity of components in the solution LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. No. L32250) is proprietary.

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The concentrations of Calcein, AM and SYTOX Deep Red Nucleic Acid stain in the LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red) (Cat. No. L32250) are proprietary. If you need to use a defined concentration of Calcein, AM we offer this as a standalone product (Cat. Nos. C3099 and C3100MP). If you need to use a defined concentration of SYTOX Deep Red Nucleic Acid stain we offer this as a standalone product (Cat. Nos. S11380 and S11380).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.