Nobiletin attenuates VLDL overproduction, dyslipidemia, and atherosclerosis in mice with diet-induced insulin resistance.
AuthorsMulvihill EE, Assini JM, Lee JK, Allister EM, Sutherland BG, Koppes JB, Sawyez CG, Edwards JY, Telford DE, Charbonneau A, St-Pierre P, Marette A, Huff MW,
JournalDiabetes
PubMed ID21471511
Increased plasma concentrations of apolipoprotein B100 often present in patients with insulin resistance and confer increased risk for the development of atherosclerosis. Naturally occurring polyphenolic compounds including flavonoids have antiatherogenic properties. The aim of the current study was to evaluate the effect of the polymethoxylated flavonoid nobiletin on lipoprotein secretion ... More
Fluorescent labeling of lipoproteins.
AuthorsVia DP, Smith LC
JournalMethods Enzymol
PubMed ID3724556
SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages.
AuthorsBerger SB, Romero X, Ma C, Wang G, Faubion WA, Liao G, Compeer E, Keszei M, Rameh L, Wang N, Boes M, Regueiro JR, Reinecker HC, Terhorst C,
JournalNat Immunol
PubMed ID20818396
'Phagocytosis is a pivotal process by which macrophages eliminate microorganisms after recognition by pathogen sensors. Here we unexpectedly found that the self ligand and cell surface receptor SLAM functioned not only as a costimulatory molecule but also as a microbial sensor that controlled the killing of gram-negative bacteria by macrophages. ... More
Grp78 is involved in retention of mutant low density lipoprotein receptor protein in the endoplasmic reticulum.
AuthorsJørgensen MM, Jensen ON, Holst HU, Hansen JJ, Corydon TJ, Bross P, Bolund L, Gregersen N
JournalJ Biol Chem
PubMed ID10906332
'The low density lipoprotein (LDL) receptor is responsible for removing the majority of the LDL cholesterol from the plasma. Mutations in the LDL receptor gene cause the disease familial hypercholesterolemia (FH). Approximately 50% of the mutations in the LDL receptor gene in patients with FH lead to receptor proteins that ... More
[Lipid metabolism in retinal pigment epithelium. Possible significance of lipoprotein receptors]
AuthorsNoske UM, Schmidt-Erfurth U, Meyer C, Diddens H
JournalOphthalmologe
PubMed ID10025142
'PURPOSE: The retinal pigment epithelium (RPE) regulates the lipid metabolism of the photoreceptors by catalysis of membrane outer segments and via choriocapillary perfusion is also exposed to the regulation of blood lipid levels. Since the uptake the metabolism of cholesterol are mediated by specific low-density lipoprotein (LDL) receptors, expression and ... More
Three probe flow cytometry of a human foam-cell forming macrophage.
AuthorsHassall DG
JournalCytometry
PubMed ID1526197
'A human cell line THP-1 was differentiated into macrophages expressing the scavenger receptor for uptake of modified lipoproteins. The cells were exposed to native low-density lipoprotein (n-LDL), acetylated-low-density lipoprotein (Ac-LDL), oxidised-LDL, or 25-OH cholesterol, leading to the accumulation of cholesteryl esters within the cells. Harvested macrophages were studied using three ... More
Changes in the spectral properties of a plasma membrane lipid analog during the first seconds of endocytosis in living cells.
AuthorsChen CS, Martin OC, Pagano RE
JournalBiophys J
PubMed ID8994591
'N-[5-(5, 7-dimethyl Bodipy)-1-pentanoyl]-D-erythro-sphingosylphosphorylcholine (C5-DMB-SM), a fluorescent analog of sphingomyelin, has been used in a study of the formation of very early endosomes in human skin fibroblasts. This lipid exhibits a shift in its fluorescence emission maximum from green (approximately 515 nm) to red (approximately 620 nm) wavelengths with increasing concentrations ... More
Molecular counting of low-density lipoprotein particles as individuals and small clusters on cell surfaces.
AuthorsGross D, Webb WW
JournalBiophys J
PubMed ID3719072
'We employ the intensely fluorescent analogue diI-LDL (Barak, L. S., and W. W. Webb, 1981, J. Cell Biol. 90:595-604) as a counting marker to determine the numbers of LDL-receptor complexes that are contained in clusters on the surfaces of human fibroblasts and human epidermoid carcinoma cells. The application of quantitative ... More
Regulation of caveolar endocytosis by syntaxin 6-dependent delivery of membrane components to the cell surface.
AuthorsChoudhury A, Marks DL, Proctor KM, Gould GW, Pagano RE
JournalNat Cell Biol
PubMed ID16565709
'Caveolar endocytosis has an important function in the cellular uptake of some bacterial toxins, viruses and circulating proteins. However, the molecular machinery involved in regulating caveolar uptake is poorly defined. Here, we demonstrate that caveolar endocytosis is regulated by syntaxin 6, a target membrane soluble N-ethylmaleimide attachment protein receptor (t-SNARE) ... More
Delivery of ligands from sorting endosomes to late endosomes occurs by maturation of sorting endosomes.
AuthorsDunn KW, Maxfield FR
JournalJ Cell Biol
PubMed ID1560027
'After endocytosis, lysosomally targeted ligands pass through a series of endosomal compartments. The endocytic apparatus that accomplishes this passage may be considered to take one of two forms: (a) a system in which lysosomally targeted ligands pass through preexisting, long-lived early sorting endosomes and are then selectively transported to long-lived ... More
Constrained diffusion or immobile fraction on cell surfaces: a new interpretation.
AuthorsFeder TJ, Brust-Mascher I, Slattery JP, Baird B, Webb WW
JournalBiophys J
PubMed ID8744314
'Protein lateral mobility in cell membranes is generally measured using fluorescence photobleaching recovery (FPR). Since the development of this technique, the data have been interpreted by assuming free Brownian diffusion of cell surface receptors in two dimensions, an interpretation that requires that a subset of the diffusing species remains immobile. ... More
Rapid fluorometric assay of LDL receptor activity by DiI-labeled LDL.
AuthorsStephan ZF, Yurachek EC
JournalJ Lipid Res
PubMed ID8381454
'DiI-LDL (3,3''-dioctadecylindocarbocyanine-low density lipoprotein) has been extensively used in morphological and microscopic studies of receptor-mediated metabolism of LDL in many cell lines. To date the use of this fluorescent probe in a quantitative assay of LDL receptor activity has not been widely used in studies with multiple samples due to ... More
Retroviral-mediated gene transfer in human hepatocytes.
AuthorsRaper SE, Wilson JM, Grossman M
JournalSurgery
PubMed ID1641772
'BACKGROUND. The ability to modify human hepatocytes genetically is an essential first step in the development of liver-directed ex vivo gene therapy for inherited metabolic disease. The purpose of these studies was to prove that the genome of human hepatocytes can be altered successfully to express foreign genetic material. METHODS. ... More
Expression of different lipoprotein receptors in natural killer cells and their effect on natural killer proliferative and cytotoxic activity.
AuthorsDe Sanctis JB, Blanca I, Bianco NE
JournalImmunology
PubMed ID8550077
'Natural killer (NK) cells take up chylomicrons (CM), very low density (VLDL), low density (LDL), high density (HDL) and acetyl-modified low density (AcLDL) lipoproteins through different receptors, VLDL being the lipoprotein with the highest uptake and HDL the lowest. The uptake of LDL can be selectively blocked by the anti-LDL ... More
Determination of LDL- and scavenger-receptor activity in adherent and non-adherent cultured cells with a new single-step fluorometric assay.
AuthorsTeupser D, Thiery J, Walli AK, Seidel D
JournalBiochim Biophys Acta
PubMed ID8908153
'Lipoproteins labeled with 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate (DiI) are widely used to visualize LDL-and scavenger-receptor activity in cultured cells. The purpose of this study was to evaluate a new single-step fluorometric assay with high sensitivity for the quantitative determination of the LDL- or scavenger-receptor activity in adherent and non-adherent cells. We used ... More
Effect of morphine on uptake of immunoglobulin G complexes by mesangial cells and macrophages.
AuthorsSinghal PC, Pan C, Gibbons N
JournalAm J Physiol
PubMed ID8498540
'Focal glomerular sclerosis is the predominant renal lesion in heroin addicts. We studied whether morphine, a metabolite of heroin, could directly affect the uptake of immunoglobulin G (IgG) complexes by cultured mesangial cells (MC) and macrophages (MP). Pre-incubation of morphine (10(-6) M) decreased uptake of IgG complexes [morphine, 66,577 +/- ... More
Flow cytometric evaluation of LDL receptors using DiI-LDL uptake and its application to B and T lymphocytic cell lines.
'Low density lipoprotein receptors (LDL-R) on Daudi Burkitt''s lymphoma (BL) cells were assessed using fluorescent DiI (3,3''-dioctadecylindocarbocyanine iodide)-LDL and flow cytometric analyses. Receptor-specific binding of DiI-LDL is followed by internalization of the bound complex and lysosomal hydrolysis of the ligand. Increase in the fluorescence intensity per cell is hence used ... More
Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C.
AuthorsAnderson CM, Georgiou GN, Morrison IE, Stevenson GV, Cherry RJ
JournalJ Cell Sci
PubMed ID1629253
'A fluorescence imaging system, based on using a cooled slow-scan CCD camera, has been developed for tracking receptors on the surfaces of living cells. The technique is applicable to receptors for particles such as lipoproteins and viruses that can be labeled with a few tens of fluorophores. The positions of ... More
Low temperatures and hypertonicity do not block cytokine-induced stimulation of the sphingomyelin pathway but inhibit nuclear factor-kappa B activation.
AuthorsAndrieu N, Salvayre R, Jaffrézou JP, Levade T
JournalJ Biol Chem
PubMed ID7592669
'In order to better understand the significance of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta)-receptor internalization in the sphingomyelin pathway signal transduction, we investigated receptor signaling under conditions in which receptor internalization is blocked. We demonstrate that human recombinant TNF-alpha and IL-1 beta both induced sphingomyelin and phosphatidylcholine ... More
Characterization of nodular neuronal heterotopia in children.
AuthorsHannan AJ, Servotte S, Katsnelson A, Sisodiya S, Blakemore C, Squier M, Molnár Z
JournalBrain
PubMed ID10071051
'Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal ... More
HLA-Cw3 expression on porcine endothelial cells protects against xenogeneic cytotoxicity mediated by a subset of human NK cells.
AuthorsSeebach JD, Comrack C, Germana S, LeGuern C, Sachs DH, DerSimonian H
JournalJ Immunol
PubMed ID9317166
'There is increasing evidence that NK cells make an important contribution to human anti-porcine xenogeneic cytotoxicity. Most allogeneic as well as autologous normal cells are not susceptible to NK cell-mediated cytotoxicity because they express inhibitory molecules encoded within the MHC class I loci. The protective signal is delivered to NK ... More
A new function for the LDL receptor: transcytosis of LDL across the blood-brain barrier.
AuthorsDehouck B, Fenart L, Dehouck MP, Pierce A, Torpier G, Cecchelli R
JournalJ Cell Biol
PubMed ID9265653
'Lipoprotein transport across the blood-brain barrier (BBB) is of critical importance for the delivery of essential lipids to the brain cells. The occurrence of a low density lipoprotein (LDL) receptor on the BBB has recently been demonstrated. To examine further the function of this receptor, we have shown using an ... More
The TNF-like protein 1A-death receptor 3 pathway promotes macrophage foam cell formation in vitro.
AuthorsMcLaren JE, Calder CJ, McSharry BP, Sexton K, Salter RC, Singh NN, Wilkinson GW, Wang EC, Ramji DP,
JournalJ Immunol
PubMed ID20410491
'TNF-like protein 1A (TL1A), a TNF superfamily cytokine that binds to death receptor 3 (DR3), is highly expressed in macrophage foam cell-rich regions of atherosclerotic plaques, although its role in foam cell formation has yet to be elucidated. We investigated whether TL1A can directly stimulate macrophage foam cell formation in ... More
Correction of the genetic defect in hepatocytes from the Watanabe heritable hyperlipidemic rabbit.
AuthorsWilson JM, Johnston DE, Jefferson DM, Mulligan RC
JournalProc Natl Acad Sci U S A
PubMed ID2454468
'Familial hypercholesterolemia is an inherited disease in humans that is caused by a defect in the receptor for low density lipoproteins (LDLR). The existence of an animal model for this disease, the Watanabe heritable hyperlipidemic (WHHL) rabbit, makes it an attractive candidate for developing new therapies that involve gene transfer ... More
Macrophages adhere to glucose-modified basement membrane collagen IV via their scavenger receptors.
Authorsel Khoury J, Thomas CA, Loike JD, Hickman SE, Cao L, Silverstein SC
JournalJ Biol Chem
PubMed ID8144597
'Scavenger receptors have been reported to mediate macrophage adhesion to serum-coated plastic surfaces. We report here that scavenger receptors promote the divalent cation independent adhesion of human monocytes and macrophages to surfaces coated with non-enzymatically glycated collagen IV but not to surfaces coated with native collagen IV. Ligands for scavenger ... More
Analysis of receptor clustering on cell surfaces by imaging fluorescent particles.
AuthorsMorrison IE, Anderson CM, Georgiou GN, Stevenson GV, Cherry RJ
JournalBiophys J
PubMed ID7811943
'Fluorescently labeled low density lipoproteins (LDL) and influenza virus particles were bound to the surface of human fibroblasts and imaged with a cooled slow-scan CCD camera attached to a fluorescence microscope. Particles were also imaged after attachment to polylysine-coated microscope slides. The digital images were analyzed by fitting data points ... More
Glycated phosphatidylethanolamine promotes macrophage uptake of low density lipoprotein and accumulation of cholesteryl esters and triacylglycerols.
AuthorsRavandi A, Kuksis A, Shaikh NA
JournalJ Biol Chem
PubMed ID10347212
'Non-enzymatic glycation of low density lipoprotein (LDL) has been suggested to be responsible for the increase in susceptibility to atherogenesis of diabetic individuals. Although the association of lipid glycation with this process has been investigated, the effect of specific lipid glycation products on LDL metabolism has not been addressed. This ... More
Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules.
AuthorsGhosh RN, Webb WW
JournalBiophys J
PubMed ID8061186
'We have developed a technique to detect, recognize, and track each individual low density lipoprotein receptor (LDL-R) molecule and small receptor clusters on the surface of human skin fibroblasts. Molecular recognition and high precision (30 nm) simultaneous automatic tracking of all of the individual receptors in the cell surface population ... More
Endocytosed beta-VLDL and LDL are delivered to different intracellular vesicles in mouse peritoneal macrophages.
AuthorsTabas I, Lim S, Xu XX, Maxfield FR
JournalJ Cell Biol
PubMed ID2391369
'Hypercholesterolemic rabbit beta-VLDL and human LDL are both internalized by mouse peritoneal macrophages by receptor-mediated endocytosis. However, only beta-VLDL (which binds to the cells with a much higher affinity than LDL) markedly stimulates acyl-CoA/cholesterol acyl transferase (ACAT) and induces foam cell formation in these cells. As an initial step to ... More
The End2 mutation in CHO cells slows the exit of transferrin receptors from the recycling compartment but bulk membrane recycling is unaffected.
AuthorsPresley JF, Mayor S, Dunn KW, Johnson LS, McGraw TE, Maxfield FR
JournalJ Cell Biol
PubMed ID8376460
'We have characterized a new CHO cell line (12-4) derived from a parental line, TRVb-1, that expresses the human transferrin receptor. This mutant belongs to the end2 complementation group of endocytosis mutants. Like other end2 mutants, the endosomes in 12-4 cells show a partial acidification defect. These cells internalize LDL ... More
Characterization of human low density lipoprotein binding proteins on the surface of schistosomula of Schistosoma mansoni.
AuthorsXu X, Caulfield JP
JournalEur J Cell Biol
PubMed ID1324838
'Low density lipoproteins (LDL) bound to the surface of Schistosoma mansoni may protect the parasite from assault by the immune system and provide essential lipids for the parasite in human schistosomiasis. Here we have characterized the LDL binding sites on the surface of schistosomula by comparing the binding of fluorescently ... More
An improved method on stimulated T-lymphocytes to functionally characterize novel and known LDLR mutations.
AuthorsRomano M, Di Taranto MD, Mirabelli P, D'Agostino MN, Iannuzzi A, Marotta G, Gentile M, Raia M, Di Noto R, Del Vecchio L, Rubba P, Fortunato G,
JournalJ Lipid Res
PubMed ID21865347
'The main causes of familial hypercholesterolemia (FH) are mutations in LDL receptor (LDLR) gene. Functional studies are necessary to demonstrate the LDLR function impairment caused by mutations and would be useful as a diagnostic tool if they allow discrimination between FH patients and controls. In order to identify the best ... More
Mechanisms of endothelin receptor subtype-specific targeting to distinct intracellular trafficking pathways.
'We recently reported that the endothelin (ET) receptor subtypes ET(A) and ET(B) are targeted to distinct intracellular destinations upon agonist stimulation (Bremnes, T., Paasche, J. D., Mehlum, A., Sandberg, C., Bremnes, B., and Attramadal, H. (2000) J. Biol. Chem. 275, 17596-17604). The ET(A) receptor was shown to follow the recycling ... More
Flow cytometric assessment of LDL receptor activity in peripheral blood mononuclear cells compared to gene mutation detection in diagnosis of heterozygous familial hypercholesterolemia.
AuthorsRaungaard B, Heath F, Brorholt-Petersen JU, Jensen HK, Faergeman O
JournalCytometry
PubMed ID10331627
'BACKGROUND: Studies indicate that human peripheral blood mononuclear cells mirror low-density lipoprotein (LDL) receptor activity of other cells in the body. To measure LDL receptor activity in patients with heterozygous familial hypercholesterolemia (FH), we prepared peripheral blood mononuclear cells from individuals with molecularly verified LDL receptor defective (Trp66-Gly mutation, n ... More
Flow cytometric assessment of effects of fluvastatin on low-density lipoprotein receptor activity in stimulated T-lymphocytes from patients with heterozygous familial hypercholesterolemia.
AuthorsRaungaard B, Brorholt-Petersen JU, Jensen HK, Faergeman O
JournalJ Clin Pharmacol
PubMed ID10761170
'To test the effects of fluvastatin on low-density lipoprotein (LDL) receptor activity in patients with heterozygous familial hypercholesterolemia, the authors measured LDL receptor activity in stimulated T-lymphocytes prepared from 34 patients before and after treatment with 40 mg fluvastatin daily for 12 weeks. Maximally induced pretreatment LDL receptor activities did ... More
Visualization of the interaction of native and modified lipoproteins with parenchymal, endothelial and Kupffer cells from human liver.
AuthorsKleinherenbrink-Stins MF, van de Boom JH, Schouten D, Roholl PJ, Niels van der Heyde M, Brouwer A, van Berkel TJ, Knook DL
JournalHepatology
PubMed ID1648542
'The interaction of low density lipoprotein, acetylated low density lipoprotein and apolipoprotein E-free high density lipoprotein with parenchymal, endothelial and Kupffer cells of human liver was visualized. For this purpose, the fluorescent phospholipid analog 1,1'-dioctadecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate was used to label the lipoproteins. The involvement of both parenchymal and nonparenchymal ... More
Movement of a fluorescent lipid label from a labeled erythrocyte membrane to an unlabeled erythrocyte membrane following electric-field-induced fusion.
AuthorsSowers AE
JournalBiophys J
PubMed ID3986281
'A short burst of electric field pulses was used to induce nearly simultaneous fusion among 50% or more of a population composed of unlabeled erythrocytes and erythrocytes labeled with the fluorescent lipid analogue DiI (1,1''-dihexadecyl-3,3,3'''',3''-tetra-methylindo carbocyanine perchlorate). Fusion products that ended in an hourglass shape were selected for analysis. The ... More
Augmentation of LDL receptor activities on lymphocytes by interleukin-2 and anti-CD3 antibody: a flow cytometric analysis.
AuthorsSuzuki K, Hara M, Kitani A, Harigai M, Norioka K, Kondo K, Hirata F, Sakata N, Kawakami M, Kawagoe M
JournalBiochim Biophys Acta
PubMed ID2306486
'Dormant lymphocytes are known to show little LDL receptor (LDL-R) activities. The present study was designed to determine whether or not LDL-R activities of lymphocytes from normal subjects were high enough to be measured by flow cytometry after the cells had been stimulated with recombinant interleukin-2 (IL-2) and anti-CD3 monoclonal ... More
Receptor-mediated endocytosis of low-density lipoprotein by cultured human glomerular cells.
AuthorsRayner HC, Horsburgh T, Brown SL, Lavender FL, Winder AF, Walls J
JournalNephron
PubMed ID2370930
'Lipoproteins might be involved in the pathogenesis of glomerular damage. Uptake of low-density lipoprotein (LDL) by cultured human glomerular cells has been studied using LDL, labelled with the fluorescent probe 1,1''-dioctadecyl-3,3,3''3''-tetramethyl-indocarbocyanine perchlorate (diI). Cells have been characterised using phase-contrast microscopy, monoclonal antibodies and lectins. Differentiated glomerular epithelial cells, epithelial-like cells ... More
Fluorescence studies of macrophage recognition and endocytosis of native and acetylated low-density lipoprotein.
AuthorsBerg KA, Berry ML, Sapareto SA, Petty HR
JournalBiochim Biophys Acta
PubMed ID3730431
'Macrophage recognition and endocytosis of 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate (diI)-labeled low-density lipoprotein (LDL) and acetyl LDL (Ac-LDL) was studied using fluorescence flow cytometry and fluorescence video intensification microscopy. RAW264 macrophages and U937 monocytes were grown in the tissue culture media in the presence and absence of LDL and Ac-LDL. Several lines of ... More
Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. I. Specific association of bindin with gel-phase phospholipid vesicles.
AuthorsGlabe CG
JournalJ Cell Biol
PubMed ID3972895
'Bindin is a 30,000-mol-wt protein of sea urchin sperm that is responsible for the specific adhesion of the sperm acrosomal process to the vitelline layer covering the egg plasma membrane during fertilization. Sulfated glycoconjugates are believed to be the egg surface receptors for bindin, but the mechanism by which bindin ... More
Expression of modified low-density lipoprotein receptors by trabecular meshwork cells.
AuthorsChang IL, Elner G, Yue YJ, Cornicelli A, Kawa JE, Elner VM
JournalCurr Eye Res
PubMed ID1802612
'We examined the incorporation of fluoresceinated low-density lipoprotein (LDL) and acetylated or acetoacetylated low-density lipoprotein (A-LDL or AA-LDL) by a number of ocular cells in culture. All the cells investigated, including bovine, monkey, human trabecular meshwork cells, human corneal endothelial cells, human corneal stromal cells and human scleral cells, took ... More
Role of heparan sulfate proteoglycans in the binding and uptake of apolipoprotein E-enriched remnant lipoproteins by cultured cells.
AuthorsJi ZS, Brecht WJ, Miranda RD, Hussain MM, Innerarity TL, Mahley RW
JournalJ Biol Chem
PubMed ID7683668
'Addition of apolipoprotein (apo) E to rabbit beta-very low density lipoproteins (beta-VLDL) has been shown to result in a marked enhancement of their binding and uptake by various cell types. Apolipoprotein E binds to lipoprotein receptors and proteoglycans. To distinguish between apoE binding to these sites, cells were treated with ... More
Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome.
AuthorsDunn KW, McGraw TE, Maxfield FR
JournalJ Cell Biol
PubMed ID2600137
'To study the fusion and separation of endocytic compartments, we have used digital image analysis to quantify the accumulation of fluorescent ligands in endosomes during continuous endocytosis for periods of 1-20 min. Fluorescently labeled transferrin (Tf) and low density lipoproteins (LDL) were used as markers of recycling receptors and lysosomally ... More
Local measurement of lateral motion in erythrocyte membranes by photobleaching technique.
AuthorsKapitza HG, Sackmann E
JournalBiochim Biophys Acta
PubMed ID7349883
'The lateral diffusion coefficients (D) of the molecular fluorescence probe 3,3''-dioctadecylindocarbocyanine iodide (DII) in the membrane of discoiderythrocyte ghosts has been measured with the photobleaching technique between 7 degrees C and 40 degrees C. A fluorescence microscope which allows bleaching experiments within small local fields (approx. 1 micron m2) at ... More
Oxidative modification of low density lipoprotein by diesel exhaust particles.
AuthorsIkeda M, Shitashige M, Yamasaki H, Sagai M, Tomita T
JournalBiol Pharm Bull
PubMed ID7550122
'Oxidized low density lipoprotein (LDL) has a variety of hazardous influences on biological systems. Oxidative modification of LDL by diesel exhaust particles (DEP) was studied in vitro to assess its in vivo health effects. DEP suspensions (1, 10 and 100 micrograms/ml) were incubated for 1 h with LDL (1 mg ... More
Lipoprotein uptake in primary cell cultures of rabbit atherosclerotic lesions. A fluorescence microscopic and flow cytometric study.
AuthorsJaakkola O, Kallioniemi OP, Nikkari T
JournalAtherosclerosis
PubMed ID3348844
'To characterize the lipoprotein metabolism of lipid-filled cells of atherosclerotic lesions, uptake of 3,3''-dioctadecylindocarbocyanine (DiI)-labelled low density lipoprotein (LDL), acetylated LDL (Ac-LDL) and beta-very low density lipoprotein (beta-VLDL) was studied by fluorescence microscopy and flow cytometry in primary cultures of enzymatically dispersed aortic cells from cholesterol-fed rabbits. Most of the ... More
Inducible nitric-oxide synthase and nitric oxide production in human fetal astrocytes and microglia. A kinetic analysis.
AuthorsDing M, St Pierre BA, Parkinson JF, Medberry P, Wong JL, Rogers NE, Ignarro LJ, Merrill JE
JournalJ Biol Chem
PubMed ID9111039
'The understanding of the induction and regulation of inducible nitric-oxide synthase (iNOS) in human cells may be important in developing therapeutic interventions for inflammatory diseases. In the present study, we not only demonstrated that human fetal mixed glial cultures, as well as enriched microglial cultures, synthesize iNOS and nitric oxide ... More
Flow cytometric assessment of LDL ligand function for detection of heterozygous familial defective apolipoprotein B-100.
'BACKGROUND: Familial defective apolipoprotein (apo) B-100 (FDB) is caused by a mutation in the apoB gene and characterized by decreased binding of LDL to LDL receptors because of reduced function of the apoB-100 ligand. FDB may be associated with severe hypercholesterolemia and cannot always be distinguished from familial hypercholesterolemia phenotypically. ... More
Human and monkey corneal endothelium expression of low-density lipoprotein receptors.
AuthorsElner SG, Elner VM, Pavilack MA, Davis HR, Cornicelli JA, Yue BY
JournalAm J Ophthalmol
PubMed ID1985497
'Receptors for low-density lipoprotein are necessary for high-affinity uptake of lipid and protein essential to cell structure and function. Distinct receptors for acetoacetylated low-density lipoprotein internalize oxidized or enzymatically modified low-density lipoprotein and extracellular matrix components. We identified low-density lipoprotein receptors on cultured human and monkey corneal endothelial cells by ... More
Identification of megalin/gp330 as a receptor for lipoprotein(a) in vitro.
AuthorsNiemeier A, Willnow T, Dieplinger H, Jacobsen C, Meyer N, Hilpert J, Beisiegel U
JournalArterioscler Thromb Vasc Biol
PubMed ID10073957
'Lipoprotein(a) [Lp(a)] is an atherogenic lipoprotein of unknown physiological function. The mechanism of Lp(a) atherogenicity as well as its catabolic pathways are only incompletely understood at present. In this report, we show that the low density lipoprotein receptor (LDLR) gene family member megalin/glycoprotein (gp) 330 is capable of binding and ... More
Identification of scavenger receptor SR-BI as a high density lipoprotein receptor.
AuthorsActon S, Rigotti A, Landschulz KT, Xu S, Hobbs HH, Krieger M
JournalScience
PubMed ID8560269
'High density lipoprotein (HDL) and low density lipoprotein (LDL) are cholesterol transport particles whose plasma concentrations are directly (LDL) and inversely (HDL) correlated with risk for atherosclerosis. LDL catabolism involves cellular uptake and degradation of the entire particle by a well-characterized receptor. HDL, in contrast, selectively delivers its cholesterol, but ... More
The mysteries of lipoprotein(a).
AuthorsUtermann G
JournalScience
PubMed ID2530631
'Lipoprotein(a) [Lp(a)] is a macromolecular complex found in human plasma that combines structural elements from the lipoprotein and blood clotting systems and that is associated with premature coronary heart disease and stroke. It is assembled from low-density lipoprotein (LDL) and a large hydrophilic glycoprotein called apolipoprotein(a) [apo(a)], which is homologous ... More
Specific binding of human low-density lipoprotein to the surface of schistosomula of Schistosoma mansoni and ingestion by the parasite.
AuthorsBennett MW, Caulfield JP
JournalAm J Pathol
PubMed ID2024706
'Low-density lipoproteins (LDL) may be important in human schistosomiasis because LDL bound to the surface of the parasite inhibits the binding of anti-schistosomal antibodies. Low-density lipoproteins also may serve as a source of lipids for the parasite membrane synthesis. Here LDL fluorescently labeled with carbocyanine dye (DiI-LDL) was used to ... More
RNA polymerase clamps down.
AuthorsLandick R
JournalCell
PubMed ID11389826
Digital imaging fluorescence microscopy.
AuthorsSmith LC, Benson DM, Gotto AM, Bryan J
JournalMethods Enzymol
PubMed ID3724557
Receptor-mediated endocytosis of low-density lipoprotein in cultured cells.
AuthorsGoldstein JL, Basu SK, Brown MS
JournalMethods Enzymol
PubMed ID6321901
Metabolism of lipoproteins labeled with fluorescent indocarbocyanine dyes for in vitro studies of atherogenesis.
AuthorsReynolds GD
JournalSurv Synth Pathol Res
PubMed ID3939385
Diffusion of low density lipoprotein-receptor complex on human fibroblasts.
AuthorsBarak LS, Webb WW
JournalJ Cell Biol
PubMed ID6296157
Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells.
Authors Matsui H; Johnson L G; Randell S H; Boucher R C;
JournalJ Biol Chem
PubMed ID8995411
The target cells for gene therapy of cystic fibrosis lung disease are the well differentiated cells that line airway lumens. Employing cultures of airway epithelial cells that grow like ... More
Generation of superoxide anion by brain endothelial cell xanthine oxidase.
AuthorsTerada LS, Willingham IR, Rosandich ME, Leff JA, Kindt GW, Repine JE
JournalJ Cell Physiol
PubMed ID1652587
Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased (greater than 20-fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O2-) into the extracellular medium. Treatment of EC with tungsten decreased (P less ... More
Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor.
AuthorsPolvino WJ, Dichek DA, Mason J, Anderson WF
JournalSomat Cell Mol Genet
PubMed ID1475710
The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length ... More
FH-Pyrgos: a novel mutation in the promoter (-45delT) of the low-density lipoprotein receptor gene associated with familial hypercholesterolemia.
AuthorsDedoussis GV, Pitsavos C, Kelberman D, Skoumas J, Prassa ME, Choumerianou DM, Stefanadis C, Humphries SE, Toutouzas P
JournalClin Genet
PubMed ID14616764
In a patient with familial hypercholesterolemia (FH), we have identified a new mutation (-45delT) in repeat 3 of the low-density lipoprotein receptor (LDLR) gene promoter. Analysis of a neutral polymorphism in the LDLR mRNA from the patient's white blood cells showed that the expression of one allele was significantly reduced, ... More
Synaptotagmin regulation of coated pit assembly.
Authorsvon Poser C, Zhang JZ, Mineo C, Ding W, Ying Y, Sudhof TC, Anderson RG
JournalJ Biol Chem
PubMed ID10906143
Synaptotagmins bind clathrin AP-2 with high affinity via their second C(2) domain, which indicates they are involved in coated pit function. We now report that expression of synaptotagmins lacking either the second C(2) domain or the entire cytoplasmic region potently inhibit endocytosis. Inhibition was dependent on two intramembrane cysteine residues ... More
CD36 is a receptor for oxidized high density lipoprotein: implications for the development of atherosclerosis.
Atherosclerotic plaques result from the excessive deposition of cholesterol esters derived from lipoproteins and lipoprotein fragments. Tissue macrophage within the intimal space of major arterial vessels have been shown to play an important role in this process. We demonstrate in a transfection system using two human cell lines that the ... More
Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Aß uptake and degradation by astrocytes.
AuthorsBasak JM, Verghese PB, Yoon H, Kim J, Holtzman DM,
JournalJ Biol Chem
PubMed ID22383525
Accumulation of the amyloid ß (Aß) peptide within the brain is hypothesized to be one of the main causes underlying the pathogenic events that occur in Alzheimer disease (AD). Consequently, identifying pathways by which Aß is cleared from the brain is crucial for better understanding of the disease pathogenesis and ... More
Type II transforming growth factor-beta receptor recycling is dependent upon the clathrin adaptor protein Dab2.
AuthorsPenheiter SG, Singh RD, Repellin CE, Wilkes MC, Edens M, Howe PH, Pagano RE, Leof EB,
JournalMol Biol Cell
PubMed ID20881059
Transforming growth factor (TGF)-ß family proteins form heteromeric complexes with transmembrane serine/threonine kinases referred to as type I and type II receptors. Ligand binding initiates a signaling cascade that generates a variety of cell type-specific phenotypes. Whereas numerous studies have investigated the regulatory activities controlling TGF-ß signaling, there is relatively ... More
Effects of the prosegment and pH on the activity of PCSK9: evidence for additional processing events.
PCSK9, a target for the treatment of dyslipidemia, enhances the degradation of the LDL receptor (LDLR) in endosomes/lysosomes, up-regulating LDL-cholesterol levels. Whereas the targeting and degradation of the PCSK9-LDLR complex are under scrutiny, the roles of the N- and C-terminal domains of PCSK9 are unknown. Although autocatalytic zymogen processing of ... More
Identification of heterogeneous nuclear ribonucleoprotein K as a transactivator for human low density lipoprotein receptor gene transcription.
AuthorsLi H, Liu J,
JournalJ Biol Chem
PubMed ID20371611
hnRNP K, a member of the family of heterogeneous ribonucleoproteins, is known to exert various functional roles in the nucleus, cytoplasm, and mitochondria to affect different cellular processes including chromatin remodeling, transcription, splicing, and translation. Here we report, for the first time, that hnRNP K is specifically involved in human ... More
Beta2 integrins modulate the initiation and progression of atherosclerosis in low-density lipoprotein receptor knockout mice.
AuthorsMerched A, Tollefson K, Chan L,
JournalCardiovasc Res
PubMed ID19843511
Beta2 integrin-mediated adhesion is thought to be a key event in cardiovascular disease. However, results of clinical trials targeting these molecules have been disappointing. Here, we investigated the effect of inactivation of beta2 integrins at different stages of atherosclerosis by timed bone marrow transplantation (BMT) of CD18(-/-) cells in low-density ... More
Microengineered liver tissues for drug testing.
AuthorsKhetani SR, Berger DR, Ballinger KR, Davidson MD, Lin C, Ware BR,
Journal
PubMed ID25617027
Drug-induced liver injury (DILI) is a leading cause of drug attrition. Significant and well-documented differences between animals and humans in liver pathways now necessitate the use of human-relevant in vitro liver models for testing new chemical entities during preclinical drug development. Consequently, several human liver models with various levels of ... More
Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.
AuthorsBitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
JournalHum Mutat
PubMed ID19623537
Dynamin 2 (DNM2) is a large GTPase involved in the release of nascent vesicles during endocytosis and intracellular membrane trafficking. Distinct DNM2 mutations, affecting the middle domain (MD) and the Pleckstrin homology domain (PH), have been identified in autosomal dominant centronuclear myopathy (CNM) and in the intermediate and axonal forms ... More
Simple method for comparing large numbers of flow cytometry histograms exemplified by analysis of the CD4 (T4) antigen and LDL receptor on human peripheral blood lymphocytes.
AuthorsTraill KN, Böck G, Winter U, Hilchenbach M, Jürgens G, Wick G
JournalJ Histochem Cytochem
PubMed ID2426348
We have developed a simple method for comparing the relative fluorescence intensity (FI) of flow cytometry histograms. It entails assessment of the FI (equivalent to the fluorescence-activated cell sorter (FACS) channel) of the 50th or 75th percentiles of either positively stained cells or the total cell population. We illustrate the ... More
Functional characterization of two low density lipoprotein receptor gene mutations by fluorescence flow cytometric assessment of receptor activity in stimulated human T-lymphocytes.
AuthorsRaungaard B, Jensen HK, Brorholt-Petersen JU, Heath F, Faergeman O
JournalClin Genet
PubMed ID10735631
We report a functional characterization of the W23X and W66G low density lipoprotein (LDL) receptor gene mutations. The authors used two-color fluorescence flow cytometry to measure LDL receptor activity in stimulated T-lymphocytes, prepared from patients heterozygous for the W23X or W66G mutation, and compared the results with measurements of LDL ... More
Increased uptake of monocyte-treated low density lipoproteins by aortic endothelium in vivo.
AuthorsGörög P, Kakkar VV
JournalAtherosclerosis
PubMed ID3038135
A new technique was elaborated for measuring LDL uptake by rat aortic endothelial cells in vivo, using a fluorescent marker (Dil)-labelled LDL and quantifying the fluorescence in cells selectively removed from the aorta. This technique was used to study the endothelial uptake of LDL modified by activated human monocytes (LDL-A) ... More
Lovastatin increases surface low density lipoprotein receptor expression by retarding the receptor internalization rate in proliferating lymphocytes.
AuthorsChan PC, Lafrenière R, Parsons HG
JournalBiochem Biophys Res Commun
PubMed ID9196047
We examined the effects of Lovastatin on LDL receptor (LDL-R) expression and rate of internalization in interleukin-2 (IL-2) expanded phytohemagglutinin-stimulated lymphocytes. Lovastatin increased the surface LDL-R expression, but not DiI-LDL uptake, by up to 30% regardless of whether cell proliferation was affected. It caused a dose-dependent reduction in the LDL-R ... More
Evidence to support the cellular mechanism involved in serum IgG homeostasis in humans.
AuthorsWard ES, Zhou J, Ghetie V, Ober RJ
JournalInt Immunol
PubMed ID12578848
IgG is the most abundant serum antibody and is an essential component of the humoral immune response. It is known that the 'neonatal' Fc receptor (FcRn) plays a role in maintaining constant serum IgG levels by acting as a protective receptor which binds and salvages IgG from degradation. However, the ... More
Rab conversion as a mechanism of progression from early to late endosomes.
AuthorsRink J, Ghigo E, Kalaidzidis Y, Zerial M
JournalCell
PubMed ID16143105
The mechanisms of endosome biogenesis and maintenance are largely unknown. The small GTPases Rab 5 and Rab 7 are key determinants of early and late endosomes, organizing effector proteins into specific membrane subdomains. Whether such Rab machineries are indefinitely maintained on membranes or can disassemble in the course of cargo ... More
A rapid flow cytometric assay for low-density lipoprotein receptors in human peripheral blood mononuclear cells.
AuthorsRanganathan S, Hattori H, Kashyap ML
JournalJ Lab Clin Med
PubMed ID7706903
Receptor-dependent uptake of low density lipoproteins (LDLs) labeled with the fluorescent probe 3,3'-dioctadecylindocarbocyanine iodide (DiI) by human lymphocytes was measured by flow cytometry. Freshly isolated lymphocytes showed very little uptake, whereas the cells cultured in lipoprotein-free medium for 48 hours showed about a 10-fold increase in the uptake of DiI-LDL. ... More
Restoration of LDL receptor activity in mutant cells by intercellular junctional communication.
AuthorsHobbie L, Kingsley DM, Kozarsky KF, Jackman RW, Krieger M
JournalScience
PubMed ID3798096
Exchange of small molecules between cells through intercellular junctions is a widespread phenomenon implicated in many physiological and developmental processes. This type of intercellular communication can restore the activity of low-density lipoprotein (LDL) receptors in mammalian cells that are deficient in the enzyme UDP-Gal/UDP-GalNAc 4-epimerase. Pure cultures of the 4-epimerase ... More
The effects of a shear flow on the uptake of LDL and acetylated LDL by an EC monoculture and an EC-SMC coculture.
AuthorsNiwa K, Kado T, Sakai J, Karino T
JournalAnn Biomed Eng
PubMed ID15117027
To elucidate the mechanisms of localized genesis and development of atherosclerosis and anastomotic intimal hyperplasia in man, a coculture of bovine aortic endothelial cells (ECs) and smooth muscle cells (SMCs) was prepared, and the effects of a shear flow on the uptake of lipoproteins by the cells was studied by ... More
Analysis of fluorescent low density lipoprotein uptake by lymphocytes. Paradoxical increase in the elderly.
AuthorsTraill KN, Jürgens G, Böck G, Huber L, Schönitzer D, Widhalm K, Winter U, Wick G
JournalMech Ageing Dev
PubMed ID3431165
Uptake of dioctadecylindocarbocyanine (DiI)-labelled low density lipoproteins (LDL) by peripheral blood lymphocytes (PBL) from young healthy donors has been characterized by flow cytometric analysis. The receptor positive cells were primarily (greater than 70%) T cells. Saturation and competition studies were performed with freshly isolated PBL as well as after a ... More
Impact of different low-density lipoprotein (LDL) receptor mutations on the ability of LDL to support lymphocyte proliferation.
AuthorsMartínez-Botas J, Suárez Y, Reshef A, Carrero P, Ortega H, Gómez-Coronado D, Teruel JL, Leitersdorf E, Lasunción MA
JournalMetabolism
PubMed ID10421221
Based on the demand for cholesterol for membrane formation, we determined the ability of low-density lipoprotein (LDL) to support proliferation in lymphocytes bearing different LDL receptor mutations, which were treated "in vitro" with lovastatin to inhibit endogenous cholesterol synthesis. Peripheral lymphocytes were isolated from two patients with homozygous familial hypercholesterolemia ... More
Characterization of human monocytic cell line, U937, in taking up acetylated low-density lipoprotein and cholesteryl ester accumulation. A flow cytometric and HPLC study.
AuthorsSuzuki K, Sakata N, Kitani A, Hara M, Hirose T, Hirose W, Norioka K, Harigai M, Kawagoe M, Nakamura H
JournalBiochim Biophys Acta
PubMed ID2302423
The uptake of LDL and acetylated LDL and the ability of cholesteryl ester accumulation by cells of a human monocytic cell line, U937, has been characterized by flow cytometric assay using a fluorescent probe, DiI, and by high-performance liquid chromatography (HPLC). The increase of mean fluorescence intensity of U937 incubated ... More
Characterization of rapid membrane internalization and recycling.
AuthorsHao M, Maxfield FR
JournalJ Biol Chem
PubMed ID10809763
Lipids and other membrane constituents recycle between the plasma membrane and intracellular endocytic compartments. In CHO cells, approximately half of the internalized C(6)-NBD-SM, a fluorescent lipid analogue widely used as a membrane maker, recycles via the endocytic recycling compartment with a t(12) of approximately 12 min (Mayor, S., Presley, J. ... More
Low density lipoprotein binding and uptake by human and rat islet beta cells.
AuthorsGrupping AY, Cnop M, Van Schravendijk CF, Hannaert JC, Van Berkel TJ, Pipeleers DG
JournalEndocrinology
PubMed ID9322913
Abnormalities in lipoprotein metabolism are common in diabetes. It is unknown whether variations in form or concentration of lipoproteins influence the function of pancreatic beta cells. This study investigates whether low density lipoproteins (LDL) exhibit specific interactions with islet beta cells. Radioactively labeled LDL (125I-LDL) and fluorescently labeled LDL (DiI-LDL) ... More
Discovery of chemical inhibitors of the selective transfer of lipids mediated by the HDL receptor SR-BI.
AuthorsNieland TJ, Penman M, Dori L, Krieger M, Kirchhausen T
JournalProc Natl Acad Sci U S A
PubMed ID12438696
The high-density lipoprotein (HDL) receptor, scavenger receptor, class B, type I (SR-BI), mediates both the selective uptake of lipids, mainly cholesterol esters, from HDL to cells and the efflux of cholesterol from cells to lipoproteins. The mechanism underlying these lipid transfers is distinct from classic receptor-mediated endocytosis, but it remains ... More
The effects of low density lipoprotein on calcium transients in isolated rabbit cardiomyocytes.
AuthorsLiu K, Pierce GN
JournalJ Biol Chem
PubMed ID8429051
The purpose of this study was to examine the effects of low density lipoprotein (LDL) on Ca2+ transients of isolated rabbit cardiomyocytes. Incubation of cardiomyocytes with > or = 1 mg of LDL cholesterol/ml of perfusion medium induced a slow (> or = 30 min) but significant increase (2-fold) in ... More
Receptor-mediated endocytosis of low density lipoprotein: somatic cell mutants define multiple genes required for expression of surface-receptor activity.
AuthorsKingsley DM, Krieger M
JournalProc Natl Acad Sci U S A
PubMed ID6089204
We have used cell fusion and mutant reversion analysis to study a collection of Chinese hamster ovary (CHO) cell mutants that are unable to bind and internalize low density lipoprotein (LDL). Pairwise cell fusions show that these LDL receptor-deficient mutants fall into three recessive complementation groups, ldlA, ldlB, and ldlC. ... More
A novel model system for characterization of phagosomal maturation, acidification, and intracellular collagen degradation in fibroblasts.
AuthorsArora PD, Manolson MF, Downey GP, Sodek J, McCulloch CA
JournalJ Biol Chem
PubMed ID10945978
Intracellular collagen degradation by fibroblasts is an important but poorly understood pathway for the physiological remodeling of mature connective tissues. The objective of this study was to determine whether gingival fibroblasts that express endogenous alpha(2)beta(1) integrin, the collagen receptor, would exhibit the cellular machinery required for phagosomal maturation and collagen ... More
Sterol-modulated glycolipid sorting occurs in niemann-pick C1 late endosomes.
AuthorsZhang M, Dwyer NK, Neufeld EB, Love DC, Cooney A, Comly M, Patel S, Watari H, Strauss JF, Pentchev PG, Hanover JA, Blanchette-Mackie EJ
JournalJ Biol Chem
PubMed ID11032830
The Niemann-Pick C1 (NPC1) protein and endocytosed low density lipoprotein (LDL)-derived cholesterol were shown to enrich separate subsets of vesicles containing lysosomal associated membrane protein 2. Localization of Rab7 in the NPC1-containing vesicles and enrichment of lysosomal hydrolases in the cholesterol-containing vesicles confirmed that these organelles were late endosomes and ... More
Identification of intimal subendothelial cells from human aorta in primary culture.
Subendothelial cells (SEC) were obtained from the inner intimal layer of adult human aorta by collagenase treatment. SEC were identified in primary culture either as smooth muscle cells by staining with FITC-labeled antisera against human smooth muscle myosin or as macrophages, foam cells and contaminating endothelial cells by their uptake ... More
Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells.
AuthorsMuñoz S, Merlos M, Zambón D, Rodríguez C, Sabaté J, Ros E, Laguna JC
JournalJ Lipid Res
PubMed ID11734580
In a randomized, cross-over feeding trial involving 10 men with polygenic hypercholesterolemia, a control, Mediterranean-type cholesterol-lowering diet, and a diet of similar composition in which walnuts replaced approximately 35% of energy from unsaturated fat, were given for 6 weeks each. Compared with the control diet, the walnut diet reduced serum ... More
Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts.
AuthorsBarak LS, Webb WW
JournalJ Cell Biol
PubMed ID6270157
The visible wavelength excited fluorophore 3,3'-dioctadecylindocarbocyanine iodide (Dil[3]) was incorporated into human low density lipoprotein (LDL) to form the highly fluorescent LDL derivative dil(3)-LDL. Dil(3)-LDL binds to normal human fibroblasts and to human fibroblasts defective in LDL receptor internalization but does not bind to LDL receptor-negative human fibroblasts at 4 ... More
Three types of low density lipoprotein receptor-deficient mutant have pleiotropic defects in the synthesis of N-linked, O-linked, and lipid-linked carbohydrate chains.
AuthorsKingsley DM, Kozarsky KF, Segal M, Krieger M
JournalJ Cell Biol
PubMed ID3700466
Biochemical, immunological, and genetic techniques were used to investigate the genetic defects in three types of low density lipoprotein (LDL) receptor-deficient hamster cells. The previously isolated ldlB, ldlC, and ldlD mutants all synthesized essentially normal amounts of a 125,000-D precursor form of the LDL receptor, but were unable to process ... More
LDL and acetyl-LDL inhibit the NK activity and are taken up by CD56+ lymphocytes.
AuthorsJuompan L, Fournié GJ, Benoist H
JournalBiochim Biophys Acta
PubMed ID7524682
The effect of LDL and modified LDL (acetyl-LDL) was studied on human natural killer cell-mediated cytotoxicity against K562 cells. Incubation for 24 h of peripheral blood lymphocytes (PBL) with a high concentration (200 micrograms/ml) of LDL decreased the NK activity in some donors. After acetylation of the LDL protein (apoB), ... More
Standardization of a flow cytometric method for measurement of low-density lipoprotein receptor activity on blood mononuclear cells.
AuthorsLøhne K, Urdal P, Leren TP, Tonstad S, Ose L
JournalCytometry
PubMed ID7587716
Flow cytometric methods for measurement of low-density lipoprotein (LDL) receptor activity on peripheral blood mononuclear cells (PBMC) may be used to identify patients with familial hypercholesterolemia (FH). However, cellular LDL receptor activities measured in FH heterozygotes may overlap with those of healthy subjects. Analytical variation is probably responsible for some ... More
Ceramide as a modulator of endocytosis.
AuthorsChen CS, Rosenwald AG, Pagano RE
JournalJ Biol Chem
PubMed ID7768929
The effects of ceramide analogs on the uptake of markers for fluid-phase (horseradish peroxidase, HRP) and receptor-mediated (low density lipoprotein, LDL) endocytosis were studied in Chinese hamster fibroblasts. N-Hexanoyl-D-erythro-sphingosine (C6-Cer) decreased the uptake of HRP in a dose-dependent manner. Internalization was inhibited > 40% with 25 microM C6-Cer, relative to ... More
Internalization of indium-labeled LDL through a lipid chelating anchor in human pancreatic-cancer cells as a potential radiopharmaceutical for tumor localization.
AuthorsUrizzi P, Souchard JP, Palevody C, Ratovo G, Hollande E, Nepveu F
JournalInt J Cancer
PubMed ID9033634
Low-density lipoproteins (LDL) labeled with indium via a lipid-chelating agent, the bis(stearylamide) of diethylenetri-aminepentaacetic acid (L), were evaluated as a potential radiopharmaceutical (111In-L-LDL) for tumor localization by studying their internalization in human pancreatic cancer cells (Capan-1). Using Dil-LDL (1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate-LDL), this cell line was shown to bind human LDL with ... More
Clathrin-dependent and -independent internalization of plasma membrane sphingolipids initiates two Golgi targeting pathways.
AuthorsPuri V, Watanabe R, Singh RD, Dominguez M, Brown JC, Wheatley CL, Marks DL, Pagano RE
JournalJ Cell Biol
PubMed ID11481344
Sphingolipids (SLs) are plasma membrane constituents in eukaryotic cells which play important roles in a wide variety of cellular functions. However, little is known about the mechanisms of their internalization from the plasma membrane or subsequent intracellular targeting. We have begun to study these issues in human skin fibroblasts using ... More