LIVE/DEAD® FungaLight™ Yeast Viability Kit, for flow cytometry

Description

The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Specifications

Compatible Cells:	Yeast Cells
For Use With (Equipment):	Flow Cytometer
Detection Method:	Fluorescent
Format:	Tube(s)
Excitation⁄Emission (nm):	PI: 535⁄617, SYTO® 9: 485⁄498
Flow Cytometer Laser Lines:	488
Form:	Solution
Label or Dye:	SYTO® 9, propidium iodide
Number of Reactions:	200
Product Line:	FungaLight™, LIVE/DEAD®
Product Size:	1 kit
Solubility:	DMSO (Dimethylsulfoxide)
Shipping Condition:	Room Temperature

Contents & storage