LIVE/DEAD® FungaLight™ Yeast Viability Kit, for flow cytometry

Catalog number: L34952

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Flow Cytometry | Microbiological Analysis

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Description

The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Compatible Cells: Yeast Cells
For Use With (Equipment): Flow Cytometer
Detection Method: Fluorescent
Format: Tube(s)
Form: Solution
Solubility: DMSO (Dimethylsulfoxide)
Label or Dye: SYTO® 9, propidium iodide
Product Size: 1 kit
Number of Reactions: 200
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): PI: 535⁄617, SYTO® 9: 485⁄498
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of SYTO® 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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