Invitrogen™

LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Catalog number: L34952
Invitrogen™

LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Catalog number: L34952
Catalog Number
L34952
Unit Size
1 kit
Price (USD)
Price: 570.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L349521 kit
Price: 570.00
Your Price:
-
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The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability
Impermeant, Permeant
Color
Green, Red
Description
LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
Detection Method
Fluorescence
For Use With (Application)
Viability Assay
For Use With (Equipment)
Flow Cytometer
Product Type
Yeast Viability Kit
Dye Type
SYTO™ 9, propidium iodide
Emission
498/617 nm
Excitation Wavelength Range
485/535 nm
Form
Solution
Format
Tube(s)
Product Line
FungaLight™, LIVE/DEAD™
Quantity
200 Assays
Shipping Condition
Room Temperature

Contents & Storage

Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5°C to -30°C) and protect from light.