Invitrogen™
LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
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Description
The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Compatible Cells: | Yeast Cells |
|---|---|
| For Use With (Equipment): | Flow Cytometer |
| Detection Method: | Fluorescent |
| Format: | Tube(s) |
| Excitation⁄Emission (nm): | PI: 535⁄617, SYTO® 9: 485⁄498 |
| Flow Cytometer Laser Lines: | 488 |
| Form: | Solution |
| Label or Dye: | SYTO® 9, propidium iodide |
| Number of Reactions: | 200 |
| Product Line: | FungaLight™, LIVE/DEAD® |
| Product Size: | 1 kit |
| Solubility: | DMSO (Dimethylsulfoxide) |
| Shipping Condition: | Room Temperature |
Contents & storage
Contains 1 vial of SYTO® 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).
Store in freezer (-5 to -30°C) and protect from light.
Store in freezer (-5 to -30°C) and protect from light.
