Invitrogen™

LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation

Catalog number: L34967
Invitrogen™

LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation

Catalog number: L34967
Catalog Number
L34967
also known as L-34967
Unit Size
80 assays
Price (USD)
115.00
Your Price:
Availability
***
Quantity
Catalog Number
L34959
also known as L-34959
Unit Size
200 assays
Price (USD)
298.00
Your Price:
Availability
***
Quantity
Catalog Number
L34968
also known as L-34968
Unit Size
400 assays
Price (USD)
329.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
L34967
also known as L-34967
80 assays
115.00
Your Price:
***
L34959
also known as L-34959
200 assays
298.00
Your Price:
***
L34968
also known as L-34968
400 assays
329.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
The LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer.

• Stable—dyes are freeze dried in separate vials to maintain stability

• Robust—staining pattern is the same before and after fixation

• Low compensation—minimal spectral overlap between other fluorophores

View a selection guide for all fixable viability dyes for flow cytometry.

Stable
Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Yellow Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.

Robust
Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Low compensation
The LIVE/DEAD™ Fixable Yellow Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser. The yellow-fluorescent reactive dye has an excitation maximum of ∼405 nm, but it is excited well with the 405 nm violet laser, and it has an emission maxima of ∼570 nm, so it can be collected in the second or third channel on most violet laser flow cytometers.

How it works
In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available
LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Eukaryotic Cells
Detection Method
Fluorescent
Dye Type
LIVE/DEAD™ Fixable Yellow Dead Cell Stain
Emission
405/575
Form
Solid
Format
Tube(s)
Product Line
LIVE/DEAD™
Quantity
80 assays
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
For Use With (Equipment)
Flow Cytometer

Contents & Storage

Contains 2 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO. Store at -20°C.

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Frequently asked questions (FAQs)

Citations & References