LIVE <i>Bac</i>Light™ Bacterial Gram Stain Kit, for microscopy & quantitative assays

Zitierungen und Referenzen (7)

Invitrogen™

LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays

Das LIVE BacLight™ Bakterielle Gram-Färbekit liefert einen neuartigen Fluoreszenz-Assay in einem Schritt zur Bestimmung des Gramsignals in lebenden Bakterien. WennWeitere Informationen

Katalognummer	Menge
L7005	1 Kit

Katalognummer L7005

Preis (EUR)

972,00

Menge:

1 Kit

Preis (EUR)

972,00

Das LIVE BacLight™ Bakterielle Gram-Färbekit liefert einen neuartigen Fluoreszenz-Assay in einem Schritt zur Bestimmung des Gramsignals in lebenden Bakterien. Wenn gramnegative und grampositive Bakterien gleichzeitig mit dem membranperativen SYTO® 9 Färbemittel und Hexidiumiodid gefärbt werden, fluoreszieren gramnegative Bakterien grün und grampositive Bakterien rot.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

BeschreibungLIVE BacLight™ Bakterien-Gram-Färbemittelkit für Mikroskopie und quantitative Assays

NachweisverfahrenFluoreszenz, Fluoreszent

FarbstofftypAndere Etiketten oder Farbstoffe

FormatRöhrchen, 96 Well-Platte, Objektträger, Röhrchen, 96-Well-Platte, Objektträger

Menge1 Kit

VersandbedingungRaumtemperatur, Raumtemperatur

FarbeGrün, rot

Emission480 bis 700 nm

Excitation Wavelength Range470 nm

Zur Verwendung mit (Anwendung)Viabilitätsassay

Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Fluorometer, Durchflusszytometer, Mikrotiterplatten-Lesegerät, Fluoreszenzmikroskop, Fluorometer, Durchflusszytometer, Mikrotiterplatten-Lesegerät

ProduktlinieBacLight

ProdukttypBakterien-Färbemittelkit

Unit SizeEach

Inhalt und Lagerung

In einem Tiefkühlgerät (-5 bis -30 °C) lagern und vor Licht schützen.

Häufig gestellte Fragen (FAQ)

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the excitation/emission maxima for SYTO 9 stain and hexidium iodide?

The excitation/emission maxima for these dyes are 480/500 nm for SYTO 9 stain and 480/625 nm for hexidium iodide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Zitierungen und Referenzen (7)

Zitierungen und Referenzen

Abstract

Persistence and cell culturability of biocontrol strain Pseudomonas fluorescens CHA0 under plough pan conditions in soil and influence of the anaerobic regulator gene anr.

Authors:Mascher F, Schnider-Keel U, Haas D, Défago G, Moënne-Loccoz Y

Journal:Environ Microbiol

PubMed ID:12558593

'Certain fluorescent pseudomonads can protect plants from soil-borne pathogens, and it is important to understand how these biocontrol agents survive in soil. The persistence of the biocontrol strain Pseudomonas fluorescens CHA0-Rif under plough pan conditions was assessed in non-sterile soil microcosms by counting total cells (immunofluorescence microscopy), intact cells (BacLight ... More

Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:Lebaron P, Parthuisot N, Catala P

Journal:Appl Environ Microbiol

PubMed ID:9572943

'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More

Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.

Authors:Armstrong DW, He L

Journal:Anal Chem

PubMed ID:11605830

The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. ... More

Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.

Authors:Durtschi JD, Erali M, Bromley LK, Herrmann MG, Petti CA, Smith RE, Voelkerding KV

Journal:J Med Microbiol

PubMed ID:16091435

A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects ... More

Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria.

Authors:Forster S, Snape JR, Lappin-Scott HM, Porter J

Journal:Appl Environ Microbiol

PubMed ID:12324319

Wastewater treatment is one of the most important commercial biotechnological processes, and yet the component bacterial populations and their associated metabolic activities are poorly understood. The novel fluorescent dye hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls. Differentiation between gram-positive and gram-negative wastewater bacteria ... More

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