Citas y referencias (69)

Invitrogen™

Kit de viabilidad de esperma LIVE/DEAD™

El kit de viabilidad de esperma LIVE/DEAD proporciona un nuevo ensayo basado en la fluorescencia para el análisis de laMás información

Número de catálogo	Cantidad
L7011	1 kit

Número de catálogo L7011

Precio (MXN)

Cantidad:

1 kit

El kit de viabilidad de esperma LIVE/DEAD proporciona un nuevo ensayo basado en la fluorescencia para el análisis de la viabilidad y el potencial de fertilización de los espermatozoides por microscopía de fluorescencia o citometría de flujo. El tinte de ácido nucleico permeable en membrana SYBR® 14 etiqueta el esperma vivo con fluorescente verde y el yoduro de propidio no permeable en membrana etiqueta los ácidos nucléicos de esperma con membrana comprometica con fluorescente rojo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas eucariotas, células espermáticas

DescripciónKit de viabilidad de esperma LIVE/DEAD™

Método de detecciónFluorescente

Tipo de coloranteOtras etiquetas o colorantes

FormatoTubos, portaobjetos

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

ColorVerde, rojo

Intervalo de longitud de onda de emisión516, 617 nm

Para utilizar con (aplicación)Ensayo de viabilidad

Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo

Línea de productosLIVE/DEAD

Tipo de productoKit de viabilidad de espermatozoides

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (69)

Citations & References

Abstract

Seminal plasma addition attenuates the dilution effect in bovine sperm.

Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH

Journal:Theriogenology

PubMed ID:11467516

Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More

Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.

Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV

Journal:Biol Reprod

PubMed ID:11870056

This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More

Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).

Authors:Chalah T, Brillard JP

Journal:Theriogenology

PubMed ID:10732141

'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More

Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.

Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M

Journal:Anim Reprod Sci

PubMed ID:12695055

'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More

Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.

Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R

Journal:Cytometry

PubMed ID:10918286

'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More

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